Protein Info for BWI76_RS09145 in Klebsiella michiganensis M5al

Annotation: serine-type D-Ala-D-Ala carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00768: Peptidase_S11" amino acids 32 to 266 (235 residues), 343.8 bits, see alignment E=7.7e-107 PF13354: Beta-lactamase2" amino acids 46 to 196 (151 residues), 44 bits, see alignment E=2.7e-15 PF07943: PBP5_C" amino acids 285 to 376 (92 residues), 86.3 bits, see alignment E=1.9e-28

Best Hits

Swiss-Prot: 84% identical to DACC_ECOLI: D-alanyl-D-alanine carboxypeptidase DacC (dacC) from Escherichia coli (strain K12)

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 95% identity to kpu:KP1_1832)

MetaCyc: 84% identical to D-alanyl-D-alanine carboxypeptidase DacC (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4 or 3.4.17.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1L5 at UniProt or InterPro

Protein Sequence (400 amino acids)

>BWI76_RS09145 serine-type D-Ala-D-Ala carboxypeptidase (Klebsiella michiganensis M5al)
MTHDAFSLRGLAAGCALLFLVAPAVQAAEQLPDAPSIDARAWILMDYASGKVLSEGNADE
KLDPASLTKIMTSYVVGQALKAGKIKSTDMVTIGRDAWATGNPALRGSSVMFLKPGMQVS
VEDLNKGVIIQSGNDASIAIADYVAGSQDAFVSLMNGYAQKMGLTNTTFMTVHGLDAPGQ
FSTARDMALLTKAMIHDVPEEYAIHKEKEFTFNKIRQPNRNRLLWSTNLNADGVKTGTTA
GAGYNLVSSATQNDMRLIAVVLGTKTDRIRFNESEKLLTWGFRFYETVTPIKPDATFITQ
RVWFGDSSEAKLGAGEAGSITLPKGQLKNLKASYTLNDTQLTAPLTKGQVVGTIDFKLND
KTIEQRPLIVMEAVNEGGFFSRMIDFMLMKLHGWFGGWFS