Protein Info for BWI76_RS08785 in Klebsiella michiganensis M5al

Annotation: excinuclease ABC subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 TIGR00631: excinuclease ABC subunit B" amino acids 5 to 664 (660 residues), 1107.7 bits, see alignment E=0 PF04851: ResIII" amino acids 16 to 87 (72 residues), 41 bits, see alignment E=5.8e-14 PF17757: UvrB_inter" amino acids 159 to 249 (91 residues), 110.5 bits, see alignment E=1.1e-35 PF00271: Helicase_C" amino acids 435 to 545 (111 residues), 73.3 bits, see alignment E=5.7e-24 PF12344: UvrB" amino acids 552 to 593 (42 residues), 75.3 bits, see alignment 7.7e-25 PF02151: UVR" amino acids 632 to 665 (34 residues), 28.5 bits, see alignment (E = 2.8e-10)

Best Hits

Swiss-Prot: 95% identical to UVRB_KLEP3: UvrABC system protein B (uvrB) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_14465)

MetaCyc: 95% identical to UvrABC excision nuclease subunit B (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit B" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B191 at UniProt or InterPro

Protein Sequence (671 amino acids)

>BWI76_RS08785 excinuclease ABC subunit B (Klebsiella michiganensis M5al)
MSKPFKLHSAFRPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSGKTFTIANVIADLQRPT
MVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIE
QMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLHLTVGMIIDQRAILRRLAELQ
YTRNDQAFQRGTFRVRGEVIDIFPAESDDVALRVELFDEEVERLSLFDPLTGHIESTIPR
FTVYPKTHYVTPRERIVQAMEEIKVELAERRKVLLENNKLLEEQRLSQRTQFDLEMMNEL
GYCSGIENYSRYLSGRGPGEPPPTLFDYLPADGLLVIDESHVTIPQIGGMYRGDRARKET
LVEYGFRLPSALDNRPMKFEEFEALAPQTIYVSATPGAYELDKSGDEVVDQVVRPTGLLD
PIIEVRPVGTQVDDLLSEIRLRTAINERVLVTTLTKRMAEDLTEYLEEHGERVRYLHSDI
DTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIG
RAARNINGKAILYGDKITPSMAKAIGETERRREKQQAYNEEHGIVPQGLNKKVVDILALG
QNIAKTKTKGRGKSRSPVEADTVALTPKALQQKIHELEGQMMQHAQNLEFEEAAQIRDQL
HQLRELFIAAS