Protein Info for BWI76_RS08535 in Klebsiella michiganensis M5al

Annotation: translocation protein TolB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02800: Tol-Pal system beta propeller repeat protein TolB" amino acids 15 to 427 (413 residues), 522.8 bits, see alignment E=3.2e-161 PF04052: TolB_N" amino acids 23 to 121 (99 residues), 112.2 bits, see alignment E=3.4e-36 PF07676: PD40" amino acids 203 to 223 (21 residues), 19.2 bits, see alignment (E = 2.4e-07) amino acids 237 to 272 (36 residues), 38.2 bits, see alignment 2.5e-13 amino acids 281 to 315 (35 residues), 44.8 bits, see alignment 2.2e-15 amino acids 369 to 396 (28 residues), 17.8 bits, see alignment (E = 6.6e-07) PF10647: Gmad1" amino acids 245 to 359 (115 residues), 21.3 bits, see alignment E=5.5e-08 PF00930: DPPIV_N" amino acids 248 to 349 (102 residues), 32.4 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 98% identical to TOLB_KLEP3: Tol-Pal system protein TolB (tolB) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K03641, TolB protein (inferred from 97% identity to kpu:KP1_1702)

MetaCyc: 92% identical to Tol-Pal system periplasmic protein TolB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B132 at UniProt or InterPro

Protein Sequence (430 amino acids)

>BWI76_RS08535 translocation protein TolB (Klebsiella michiganensis M5al)
MKQALRVAFGFLMLWAAVLHAEVRIEITQGVDSARPIGVVPFQWAGQGAAPEDVGGIVAA
DLRNSGKFNPLDRSRLPQQPGSAQEVQPAAWSALGVDAVVVGQVTQNPDGSYNVAYQLVD
TGGAPGTVLAQNSFKVTKQYLRYAAHAASDAVFEKLTGIKGAFRTRIAYVVQTNGGQFPY
ELRVSDYDGYNQFLVHRSTQPLMSPAWSPDGAKLAYVTFESGRSALVVQTLANGSVRQIA
SFPQHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLGSGQIRQVTNGRSNNTEPSWFPDSQ
NLAFTSDQAGRPQVYKVNINGGTPQRITWEGSQNQDADVSADGKTMVMVSSAGGQQHIAK
QDLEAGGVQVLSSTFLDETPSLAPNGTMVIYSSSQGMGSVLNLVSTDGRFKARLPATDGQ
VKSPAWSPYL