Protein Info for BWI76_RS08460 in Klebsiella michiganensis M5al

Annotation: dicarboxylate/amino acid:cation symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details transmembrane" amino acids 46 to 68 (23 residues), see Phobius details amino acids 83 to 105 (23 residues), see Phobius details amino acids 152 to 170 (19 residues), see Phobius details amino acids 190 to 211 (22 residues), see Phobius details amino acids 228 to 249 (22 residues), see Phobius details amino acids 292 to 319 (28 residues), see Phobius details amino acids 324 to 346 (23 residues), see Phobius details amino acids 353 to 360 (8 residues), see Phobius details amino acids 363 to 373 (11 residues), see Phobius details PF00375: SDF" amino acids 6 to 402 (397 residues), 349.8 bits, see alignment E=1e-108

Best Hits

Swiss-Prot: 40% identical to DCTA_AGRFC: C4-dicarboxylate transport protein (dctA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: None (inferred from 96% identity to cko:CKO_02422)

MetaCyc: 38% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"Putative symport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B114 at UniProt or InterPro

Protein Sequence (435 amino acids)

>BWI76_RS08460 dicarboxylate/amino acid:cation symporter (Klebsiella michiganensis M5al)
MKKISLTKMIILGLILGMIAGVIINNMASADTAKSYAQDISIFTTIFLRMVKMIIAPLVI
STLVVGIAKMGDAKTLGRIFSKTFFLFICASLLSIALGLVIVNLFQPGAGINFVPHDAGA
VAAVQSEPFTLKVFISHAVPTSIVDAMARNEILQIVVFSIFLGCSLAAIGEKAEPIVKVL
DSLVHVMLKLTGYVMLFAPLTVFAAISGLIAERGLGVMVSAGIFMGEFYLTLGMLWAILI
GLSTVIVGPCISRLTKAIFEPALLAFTTSSSEAAFPGTLDKLEKFGVSSKIASFVLPIGY
SFNLVGSMAYCSFATVFIAQACNVHLSMGEQITMLLILMLTSKGMAGVPRASMVVIAATL
NQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAIVGRWEGEHYGEGCRGTAPVKKP
EQDRPASETSEVAMS