Protein Info for BWI76_RS08135 in Klebsiella michiganensis M5al

Annotation: tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 4 to 440 (437 residues), 724.4 bits, see alignment E=5.3e-222 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 437 (434 residues), 521.2 bits, see alignment E=2e-160 PF00919: UPF0004" amino acids 4 to 104 (101 residues), 102.9 bits, see alignment E=1.2e-33 PF04055: Radical_SAM" amino acids 151 to 325 (175 residues), 96.2 bits, see alignment E=3.9e-31 PF01938: TRAM" amino acids 378 to 440 (63 residues), 68.7 bits, see alignment E=4.6e-23

Best Hits

Swiss-Prot: 97% identical to MIAB_CITK8: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 98% identity to cro:ROD_06741)

MetaCyc: 96% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0S8 at UniProt or InterPro

Protein Sequence (474 amino acids)

>BWI76_RS08135 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB (Klebsiella michiganensis M5al)
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVF
HQLGRWKLLKEKNPNLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
NRSPVVDISFPEIEKFDRLPEPRAEGPSAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
ILFEIAQLAAQGVREVNLLGQNVNAWRWENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDI
QISSDFIVGFPGETTQDFEQTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEDKKQ
RLYILQERINQQATAWSRRMLGTVQRILVEGTSRKSIMELSGRTENNRVVNFEGSPEMIG
KFVDVEITDVWTNSLRGKVVRTEDEMGLRIAETPESVIARTRKENDLGVGIYQP