Protein Info for BWI76_RS08075 in Klebsiella michiganensis M5al

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 transmembrane" amino acids 529 to 549 (21 residues), see Phobius details TIGR00396: leucine--tRNA ligase" amino acids 13 to 867 (855 residues), 1299.7 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 22 to 235 (214 residues), 110.8 bits, see alignment E=1.4e-35 amino acids 425 to 580 (156 residues), 32.5 bits, see alignment E=8.6e-12 amino acids 627 to 660 (34 residues), 28.5 bits, see alignment (E = 1.4e-10) PF09334: tRNA-synt_1g" amino acids 47 to 192 (146 residues), 83 bits, see alignment E=3.8e-27 amino acids 625 to 667 (43 residues), 21.2 bits, see alignment 2.3e-08 PF13603: tRNA-synt_1_2" amino acids 229 to 411 (183 residues), 259.2 bits, see alignment E=3.4e-81 PF08264: Anticodon_1" amino acids 707 to 830 (124 residues), 68.7 bits, see alignment E=1e-22

Best Hits

Swiss-Prot: 94% identical to SYL_CITK8: Leucine--tRNA ligase (leuS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_13895)

MetaCyc: 93% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0S5 at UniProt or InterPro

Protein Sequence (868 amino acids)

>BWI76_RS08075 leucine--tRNA ligase (Klebsiella michiganensis M5al)
MKTGPLAAMQEQYRPEEIESKVQQHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGH
VRNYTIGDVISRYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQL
KKLGFGYDWSREVATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVI
DGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDNLDHWPDTVKTMQRNWIGRSEGVEI
SFDVNDYADKLTVYTTRPDTFMGCTYLAVAAGHLLAQQAAANNPALATFIDECRNTKVAE
ADMATMEKKGVDTGYKAIHPLTGEAIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKY
GLNIKPVILAADGSEPDLSEQALTEKGVLFNSGEFDGLDHEAGFNAIADKLASLGVGERK
VNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPEDQLPVILPEDVVMDGITSPIKADPE
WAKTTVNGQPALRETDTFDTFMESSWYYARYTCPQYDQGMLDPEAANYWLPVDIYIGGIE
HAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPKEAT
TERDEKGRIVKAFDPQGRELVYTGMSKMSKSKNNGIDPQEMVERYGADTVRLFMMFASPA
DMTLEWQESGVEGANRFLKRVWKLVYEHTSQGEAAALDVASLSEDQKALRRDVHKTIAKV
TDDIGRRQTFNTAIAAIMELMNKLAKAPQEGEQDRALMREALLAVVRMLNPFTPHASFTL
WQELGGEGDIDNTPWPVADESAMVEDTTLVVVQVNGKVRGKITVAVDATEEQVRERAGQE
HLVAKYLDGVTVRKVIYVPGKLLNLVVG