Protein Info for BWI76_RS08025 in Klebsiella michiganensis M5al

Annotation: septal ring lytic transglycosylase RlpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF03330: DPBB_1" amino acids 78 to 166 (89 residues), 71.1 bits, see alignment E=7.5e-24 TIGR00413: rare lipoprotein A" amino acids 81 to 197 (117 residues), 96.4 bits, see alignment E=1.2e-31 PF05036: SPOR" amino acids 291 to 363 (73 residues), 56.2 bits, see alignment E=3.6e-19

Best Hits

Swiss-Prot: 77% identical to RLPA_ECOLI: Endolytic peptidoglycan transglycosylase RlpA (rlpA) from Escherichia coli (strain K12)

KEGG orthology group: K03642, rare lipoprotein A (inferred from 86% identity to kpn:KPN_00665)

Predicted SEED Role

"Rare lipoprotein A precursor" in subsystem Peptidoglycan Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0T3 at UniProt or InterPro

Protein Sequence (366 amino acids)

>BWI76_RS08025 septal ring lytic transglycosylase RlpA (Klebsiella michiganensis M5al)
MRKQWLGICIAAGLLAACSGEDVQQKTVSTPQPAVCNGPTVEISGADPQYETPNATANQD
YERDGKSYKIVQDPSNFTQAGFAAIYDAEPNSNLTASGEAFDPTQLTAAHPTLPIPSYAR
ITNLANGRMIVVRINDRGPYGNDRVISLSRASADRLNTSNNTKVRIDPIVVAPDGSLSGP
GMACTTVAKQTYALPARPNLDGDNSAGMSQPAQTDVHAISNSTLKPEDTTGAPVSSGGFL
GAPTPLNNGVLEGSEPVVQTAPVTAPGSVQGSVAPAVAATAAVTPAAAASAGGDFVVQVG
AVSDQSRAQQYQQRLSQQFSVPGRVTQNGAVWRIQLGPFSSKAQASTIQQRLQSEAQLQS
FITRAN