Protein Info for BWI76_RS07705 in Klebsiella michiganensis M5al

Annotation: enterochelin esterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 transmembrane" amino acids 279 to 296 (18 residues), see Phobius details PF11806: Enterochelin_N" amino acids 26 to 157 (132 residues), 130 bits, see alignment E=8e-42 PF00756: Esterase" amino acids 174 to 389 (216 residues), 73.9 bits, see alignment E=1.8e-24

Best Hits

Swiss-Prot: 64% identical to FES_ECOLI: Enterochelin esterase (fes) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 78% identity to eae:EAE_13605)

MetaCyc: 64% identical to ferric enterobactin esterase (Escherichia coli K-12 substr. MG1655)
RXN-20025 [EC: 3.1.1.108]; 3.1.1.108 [EC: 3.1.1.108]; 3.1.1.- [EC: 3.1.1.108]

Predicted SEED Role

"Enterobactin esterase" in subsystem Siderophore Enterobactin

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.108

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B0L2 at UniProt or InterPro

Protein Sequence (399 amino acids)

>BWI76_RS07705 enterochelin esterase (Klebsiella michiganensis M5al)
MHKTGCDDWWRTISGPQIEAVDDAYRVTFWWRDPAGDEASSATRRVWIYITGVTDHHKNA
VPQTLQRIPGTDAWRWQTTLSPTWRGSYCFIPSARDDDFSPQLFNGDGPDRALLREGWRR
LLPRAIADPLNPQSWKGGRGHAVSALELPEAPEQPGWALGEEPYQPPICIEWRSQRLGNR
RRIWVYATGDAQPQQRPLAILLDGQFWAESMPVWSPLAGLTREKRLPPAVYLLIDAIDNP
HRAAELPCNDAFWLAVQEELLPMVHRLAPFSDRADRTVVAGQSFGGLAAMFAALYWPRRF
GCVLSQSGSYWWPHRGGAQTGLLIDRLSRGELHPQGLRIWLEAGLREPIIFRANQALLAH
LEQQTIFWRQVDGGHDALCWRGGMTAGLIQLWQPLCRDE