Protein Info for BWI76_RS06945 in Klebsiella michiganensis M5al

Annotation: multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00657: Lipase_GDSL
  • amino acids 41 to 205 (165 residues), 95.1 bits, E=6.9e-31
• PF13472: Lipase_GDSL_2
  • amino acids 42 to 197 (156 residues), 105.5 bits, E=4.6e-34

Best Hits

Swiss-Prot: 89% identical to TESA_ECOLI: Thioesterase 1/protease 1/lysophospholipase L1 (tesA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_13090)

MetaCyc: 89% identical to multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (Escherichia coli K-12 substr. MG1655)
Acyl-CoA hydrolase. [EC: 3.1.2.20]; Oleoyl-[acyl-carrier-protein] hydrolase. [EC: 3.1.2.20, 3.1.2.14]; 3.1.2.14 [EC: 3.1.2.20, 3.1.2.14]; Lysophospholipase. [EC: 3.1.2.20, 3.1.2.14, 3.1.1.5]

Predicted SEED Role

"Arylesterase precursor (EC 3.1.1.2)" (EC 3.1.1.2)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (20/20 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• acyl-CoA hydrolysis (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• palmitoleate biosynthesis II (plants and bacteria) (1/2 steps found)
• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• bacterial bioluminescence (4/8 steps found)
• petroselinate biosynthesis (2/6 steps found)
• firefly bioluminescence (2/14 steps found)
• jasmonic acid biosynthesis (4/19 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Bisphenol A degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.2 or 3.1.1.5 or 3.1.2.14 or 3.1.2.20

Sequence Analysis Tools

See A0A285AZX1 at UniProt or InterPro

Protein Sequence (218 amino acids)

>BWI76_RS06945 multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1 (Klebsiella michiganensis M5al)
MLTLTDGFLKMMNFKYVFRWHLPFLFLVLFTCRAMAADTLLVLGDSLSAGYRMAGNTAWP
ALLNDKWQTKTPVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLRGFPPQQT
EQTLRTILEHIKAANAQPLLMQIRLPANYGRRYNEAFSAIYPALAKEFDIPLLPFFMEEV
YLKPQWMQDDGIHPNRDAQPFIADWMATRLAPLVNHDS