Protein Info for BWI76_RS06475 in Klebsiella michiganensis M5al
Annotation: protoheme IX farnesyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 98% identical to CYOE_KLEP7: Protoheme IX farnesyltransferase (cyoE) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
KEGG orthology group: K02301, protoheme IX farnesyltransferase [EC: 2.5.1.-] (inferred from 98% identity to kpn:KPN_00390)MetaCyc: 95% identical to heme O synthase (Escherichia coli K-12 substr. MG1655)
HEMEOSYN-RXN [EC: 2.5.1.141]
Predicted SEED Role
No annotation
MetaCyc Pathways
- heme a biosynthesis (2/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Methionine metabolism
- Porphyrin and chlorophyll metabolism
- Riboflavin metabolism
- Terpenoid biosynthesis
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.-
Use Curated BLAST to search for 2.5.1.- or 2.5.1.141
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AZV3 at UniProt or InterPro
Protein Sequence (295 amino acids)
>BWI76_RS06475 protoheme IX farnesyltransferase (Klebsiella michiganensis M5al) MFKQYLQVTKPGIIFGNLISVIGGFLLASKGHIDYPLFIWTLLGVSLVVASGCVFNNYID RDIDRKMERTKNRVLVKGLISPEASLVYATLLGIAGFMLLWFGANPLACWLGVMGFVVYV GVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGDFDSGAAILLAIFSLWQMPHSYAIA IFRFKDYQAANIPVLPVVKGISVAKNHITVYIIAFTVATLMLSLGGYAGYKYLVVAAAVS VWWLGMALRGYKVADDKVWARKLFVFSIVAITALSVMMSVDFMVPDSHNLLTMVR