Protein Info for BWI76_RS05135 in Klebsiella michiganensis M5al

Annotation: iron-hydroxamate transporter substrate-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01497: Peripla_BP_2" amino acids 38 to 267 (230 residues), 93.4 bits, see alignment E=7.6e-31

Best Hits

Swiss-Prot: 74% identical to FHUD_ECOLI: Iron(3+)-hydroxamate-binding protein FhuD (fhuD) from Escherichia coli (strain K12)

KEGG orthology group: K02016, iron complex transport system substrate-binding protein (inferred from 88% identity to kva:Kvar_4214)

MetaCyc: 74% identical to iron(III) hydroxamate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-11-RXN [EC: 7.2.2.16]; TRANS-RXN-297 [EC: 7.2.2.16]; TRANS-RXN-298 [EC: 7.2.2.16]

Predicted SEED Role

"Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), periplasmic substrate binding protein FhuD" in subsystem Iron acquisition in Vibrio or Siderophore Aerobactin (TC 3.A.1.14.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYS2 at UniProt or InterPro

Protein Sequence (296 amino acids)

>BWI76_RS05135 iron-hydroxamate transporter substrate-binding subunit (Klebsiella michiganensis M5al)
MENLTFITRRRLLTAMALSPLLWQMRGARAAEVDPQRIVALEWLPVELLLALGVTPYGVA
DIPNYRLWVNEPALPDSVIDVGLRTEPNLELLTQMKPSFLVWSAGYGPSAEKLARIAPGR
GFTFSDGKRPLMMAQQSLSEMADLIGRQQEAKRHLAEFDALMESLRPRFARRGDRPLLMI
TLLDTRHVLVFAQNCLFQEVLDRFAINNAWQGETTFWGSVTVGIDRLAAYRDADVICFDH
GNAREMAQLTATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFARVLADAQGGPA