Protein Info for BWI76_RS04885 in Klebsiella michiganensis M5al

Annotation: pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 4 to 73 (70 residues), 78.2 bits, E=1.2e-25
  • amino acids 106 to 176 (71 residues), 78 bits, E=1.4e-25
  • amino acids 206 to 276 (71 residues), 77.7 bits, E=1.8e-25
• TIGR01348: dihydrolipoyllysine-residue acetyltransferase
  • amino acids 188 to 629 (442 residues), 759.4 bits, E=1.3e-232
• PF02817: E3_binding
  • amino acids 326 to 361 (36 residues), 57.8 bits, 3.6e-19
• PF00198: 2-oxoacid_dh
  • amino acids 398 to 628 (231 residues), 270.8 bits, E=3.3e-84

Best Hits

Swiss-Prot: 90% identical to ODP2_ECOLI: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (aceF) from Escherichia coli (strain K12)

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 93% identity to esc:Entcl_3613)

MetaCyc: 90% identical to pyruvate dehydrogenase E2 subunit (Escherichia coli K-12 substr. MG1655)
Dihydrolipoyllysine-residue acetyltransferase. [EC: 1.2.1.104, 2.3.1.12]; 1.2.1.104 [EC: 1.2.1.104, 2.3.1.12]

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.12

Use Curated BLAST to search for 1.2.1.104 or 2.3.1.12

Sequence Analysis Tools

See A0A285AYV6 at UniProt or InterPro

Protein Sequence (629 amino acids)

>BWI76_RS04885 pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase (Klebsiella michiganensis M5al)
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVS
VGDKTETGKLIMIFDSADGAAAAAPAQEEKKEAAPAAAAPAAASAKEVHVPDIGGDEVEV
TEIMVKVGDTIAAEQSLITVEGDKASMEVPAPFAGTVKEIKINTGDKVSTGSLIMIFEVA
GAAPAAAPAKAEAAPAAAAPAASGSKEVHVPDIGGDEVEVTEVMVKVGDKIAAEQSLITV
EGDKASMEVPAPFAGTVKEIKISTGDKVSTGSLIMVFEVEGAAPAAAPAAAAPAPAAAPA
QAAKPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGSGRKGRILREDV
QAYVKEAVKRAEAAPAATGGGIPGMLPWPKVDFSKFGEVEEVELGRIQKISGANLSRNWV
MIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFNSSL
SEDAQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTVISKKARDGKLTAG
EMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISLSF
DHRVIDGADGARFITIINNMLSDIRRLVM