Protein Info for BWI76_RS04470 in Klebsiella michiganensis M5al

Annotation: carbamoyl phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1053 (1052 residues), 1615.5 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 7 to 125 (119 residues), 167 bits, E=7.2e-53
  • amino acids 559 to 670 (112 residues), 165.4 bits, E=2.2e-52
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 257.8 bits, E=3.7e-80
  • amino acids 674 to 876 (203 residues), 131.7 bits, E=1.4e-41
• PF02787: CPSase_L_D3
  • amino acids 427 to 505 (79 residues), 91.6 bits, 1.3e-29
• PF02655: ATP-grasp_3
  • amino acids 680 to 846 (167 residues), 29.5 bits, E=3.7e-10
• PF02222: ATP-grasp
  • amino acids 681 to 846 (166 residues), 27.4 bits, E=1.3e-09
• PF07478: Dala_Dala_lig_C
  • amino acids 699 to 844 (146 residues), 25.9 bits, E=3.3e-09
• PF02142: MGS
  • amino acids 957 to 1042 (86 residues), 74.2 bits, E=3.8e-24

Best Hits

Swiss-Prot: 97% identical to CARB_ECO57: Carbamoyl-phosphate synthase large chain (carB) from Escherichia coli O157:H7

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 73% identity to abo:ABO_0318)

MetaCyc: 97% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• allantoin degradation IV (anaerobic) (8/9 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See A0A285AYJ6 at UniProt or InterPro

Protein Sequence (1074 amino acids)

>BWI76_RS04470 carbamoyl phosphate synthase large subunit (Klebsiella michiganensis M5al)
MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM
ADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALELERQGVLAEFGVTMIGATA
DAIDKAEDRRRFDVAMKKIGLDTARSGIAHTMEEALAVAADVGFPCIIRPSFTMGGTGGG
IAYNREEFEEICERGLDLSPTNELLIDESLIGWKEYEMEVVRDKNDNCIIVCSIENFDAM
GIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFSVNPKDGRLIVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIP
RFNFEKFVGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVSLDDPEA
LTKIRRELKDAGAERIWYIADAFRAGLSVDGVFNLTNIDRWFLVQIEELVRLEEKVAEVG
ITGLDADFLRQLKRKGFADARLAKLAGVREAEIRKLRDQYDLHPVYKRVDTCAAEFATDT
AYMYSTYEDECEANPSVDRDKIMVLGGGPNRIGQGIEFDYCCVHASLALREDGYETIMVN
CNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVP
VIGTSPDAIDRAEDRERFQHAVDRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVL
GGRAMEIVYDEIDLRRYFQTAVSVSNDAPVLLDRFLDDAVEVDVDAICDGEMVLIGGIME
HIEQAGVHSGDSACSLPAYTLSQEIQDVMREQVQKLAFELQVRGLMNVQFAVKNNEVYLI
EVNPRAARTVPFVSKATGVPLAKVAARVMVGQTLAQQGVTKEIIPPYYSVKEVVLPFNKF
PGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKQGRALLSVREGDKERVVDL
AAKLLKFGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTAGR
QAIEDSKLIRRSALQYKVHYDTTLNGGFATAMALNANAMEKVTSVQEMHAQIKK