Protein Info for BWI76_RS04435 in Klebsiella michiganensis M5al

Annotation: [citrate (pro-3S)-lyase] ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 TIGR00124: [citrate (pro-3S)-lyase] ligase" amino acids 6 to 340 (335 residues), 530 bits, see alignment E=2.1e-163 PF00583: Acetyltransf_1" amino acids 21 to 102 (82 residues), 25.7 bits, see alignment E=1.8e-09 PF13508: Acetyltransf_7" amino acids 36 to 109 (74 residues), 29.4 bits, see alignment E=1.3e-10 TIGR00125: cytidyltransferase-like domain" amino acids 146 to 202 (57 residues), 42.8 bits, see alignment E=4.7e-15 PF08218: Citrate_ly_lig" amino acids 146 to 331 (186 residues), 262.1 bits, see alignment E=3.7e-82

Best Hits

Swiss-Prot: 83% identical to CITC_KLEPN: [Citrate [pro-3S]-lyase] ligase (citC) from Klebsiella pneumoniae

KEGG orthology group: K01910, [citrate (pro-3S)-lyase] ligase [EC: 6.2.1.22] (inferred from 83% identity to kpn:KPN_00034)

MetaCyc: 83% identical to [citrate [pro-3S]-lyase] ligase (Klebsiella pneumoniae)
[Citrate (pro-3S)-lyase] ligase. [EC: 6.2.1.22]

Predicted SEED Role

"[Citrate [pro-3S]-lyase] ligase (EC 6.2.1.22)" (EC 6.2.1.22)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYC2 at UniProt or InterPro

Protein Sequence (342 amino acids)

>BWI76_RS04435 [citrate (pro-3S)-lyase] ligase (Klebsiella michiganensis M5al)
MNLIIKRVTVQKDPRRREAVDLFLHQHQLSLEADCERVIVAEDQRRIVGCGAIAGCVLKC
IAVDPSLQGEGLSLKLLTELMTLAYELGRSELFLFTKPDNAALFSSAGFWPIARAGDQAV
LMENSRERLARYCRQLTMYRQPGEKIGAIVMNANPFTLGHRWLVEQAARQCDWLHLFVVK
EDASFFSYQDRVALIEQGIAGIANVTLHPGSAYLISRATFPGYFLKDKGVVDDCHCQIDL
QLFREHLAPALQITHRFVGSEPLCSLTRNYNLRMKELLEAPGETPAIQVVELERVEKDGA
PISASRVRKLYKERQWSAIAPLVPPGTLSFLMQLAESEHQTA