Protein Info for BWI76_RS03525 in Klebsiella michiganensis M5al

Annotation: tryptophanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR00233: tryptophan--tRNA ligase" amino acids 5 to 331 (327 residues), 232 bits, see alignment E=5.7e-73 PF00579: tRNA-synt_1b" amino acids 6 to 286 (281 residues), 188.6 bits, see alignment E=8.5e-60

Best Hits

Swiss-Prot: 56% identical to SYW_CLOLO: Tryptophan--tRNA ligase (trpS) from Clostridium longisporum

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 91% identity to kpe:KPK_4890)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.2

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXY2 at UniProt or InterPro

Protein Sequence (335 amino acids)

>BWI76_RS03525 tryptophanyl-tRNA synthetase (Klebsiella michiganensis M5al)
MSTSQTILTGDRPTGQLHLGHYVGSLRQRVQLQHDHQQFILVADLQGLTDNGSNPQKISS
NILEVMADYLAVGIDPQKTTICLQSALPALAELSALYMNIVTVARVERNPTVKNEIAQKG
FSRSLPVGFLAYPISQAADITAFKAELVPVGDDQLPMIEQTNEIVHKMNSLTTTPILQHC
KALLSDVSRLPGIDGNAKMSKSLGNTLTLSASEEEIHRAVSAMYTDPNHLRVADPGQIEG
NVVFTYLDAFHTDKTFVAEMKAHYQRGGLGDRQCKNALETCLQELLAPIRERRAIYIQDK
GMLLALLRRGSERAHLLTQRTLHEVKRGLGLPVLF