Protein Info for BWI76_RS03090 in Klebsiella michiganensis M5al
Updated annotation (from data): 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22)
Rationale: Specifically important for utilizing m-Inositol.
Original annotation: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to IOLD_GEOKA: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD) from Geobacillus kaustophilus (strain HTA426)
KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 94% identity to kva:Kvar_4580)MetaCyc: 56% identical to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (Bacillus subtilis subtilis 168)
RXN-14149 [EC: 3.7.1.22]
Predicted SEED Role
"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)
MetaCyc Pathways
- myo-inositol degradation I (7/7 steps found)
- myo-, chiro- and scyllo-inositol degradation (7/10 steps found)
KEGG Metabolic Maps
- 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Biphenyl degradation
- Caprolactam degradation
- Ethylbenzene degradation
- Inositol phosphate metabolism
- Limonene and pinene degradation
- Nicotinate and nicotinamide metabolism
- Phenylalanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.7.1.-
Use Curated BLAST to search for 3.7.1.- or 3.7.1.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AWX7 at UniProt or InterPro
Protein Sequence (646 amino acids)
>BWI76_RS03090 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (Klebsiella michiganensis M5al) MGKLRLTMAQALVKFLDNQYLEVDGEEHKFVKGIFAIFGHGNVLGMGQALEQDSGEMRVY QGRNEQGMAHVATGFARQSLRRQIIACTSSIGPGAANMITAAATATANRIPLLLLPGDVF ATRQPDPVLQQIEQSHDLSITTNDAFRAVSKYWDRITRPEQLMSACINAMRVLTDPAETG AVTLCLPQDVQGEAWDYPESFFTRRVHRLDRRPASAAQLADAVAAIKASRKPLIVCGGGV KYSGAGEALTRFAERYGVPFAETQAGKGSVVSSHPYNVGGVGETGCLAANLLAKEADLVI GLGTRFSDFTTSSKWIFQHPGVRFLNINVSNFDAWKLDGIPMLADAREALTALDGALSGE SWQAGWGEQIESVQSRQLKETQRVYQAVWQETAFVPEIDDHLDRESVYREFRQITDSTLT QSSVLGVLNETLPADAVIVAAAGSLPGDLQRVWRNRAANTYHVEYGYSCMGYEVSAALGV KLAQPQSEVYSLVGDGSFMMLHSELVTSLQERAKINIVLLDNMANGCINNLQMEHGMDSF GTEFRFRCAESGQLQGGLVPVDFATVAAGYGCKTWRVTTLEELRHALDAARRETVSTLID IKVLPKTMVHKYGSWWNVGVAQSALSERIRKVAQMINEKRAQARDY