Protein Info for BWI76_RS03070 in Klebsiella michiganensis M5al
Updated annotation (from data): Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27)
Rationale: Specifically important for utilizing m-Inositol. Automated validation from mutant phenotype: the predicted function (RXN-2902) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: methylmalonate-semialdehyde dehydrogenase (CoA acylating)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: None (inferred from 94% identity to eae:EAE_09795)MetaCyc: 45% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]
Predicted SEED Role
"Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27)" in subsystem Inositol catabolism (EC 1.2.1.27)
MetaCyc Pathways
- myo-inositol degradation I (7/7 steps found)
- myo-, chiro- and scyllo-inositol degradation (7/10 steps found)
- β-alanine degradation I (1/2 steps found)
- β-alanine degradation II (1/2 steps found)
- L-valine degradation I (4/8 steps found)
- propanoyl-CoA degradation II (1/5 steps found)
- 2,4-dinitrotoluene degradation (1/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.27
Use Curated BLAST to search for 1.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AWX9 at UniProt or InterPro
Protein Sequence (501 amino acids)
>BWI76_RS03070 Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) (Klebsiella michiganensis M5al) MTITGNFIGGKTVTSGSNKTMPVFDPATGKVVREVTLSTAQEVSDAIQVARDAFESWSRT TPLRRARVMFNFKMLLEQHAEELAGIIVSEHGKVFSDAMGELTRGMEVVEFACGIPHLIK GEFSSDVGTGVDSYSLMQPLGVVAGITPFNFPAMVPMWMFPLALACGNSFVLKPPALAPT AAVRLAELLKEAGLPDGVFNVVHCSNEDAEQLYRDPRIAAVSFVGSSGVAEHIYKTASAY GKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGSAGERCMALPVVVAVGDETADKLIA RLKPLVESLKVGPGCMRGKEENEMGPVVSDTHQKKVLGYIDKGVSEGATLVVDGRKPQVP GFEEGYYVGGTLFDNVTPEMTIWREEIFGPVLGIVRVADYHSALELVNSHEFGNGSAVFT SNGHTAREFVHDVQAGMVGVNVPVPVPMAFHSFGGWKRSVFGALNVHGPDGVRFYTRMKT ATVRWPQGQQTVSEFSMPTLG