Protein Info for BWI76_RS02935 in Klebsiella michiganensis M5al

Annotation: anaerobic ribonucleoside triphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 4 to 89 (86 residues), 76 bits, E=4.2e-25
• TIGR02487: anaerobic ribonucleoside-triphosphate reductase
  • amino acids 115 to 705 (591 residues), 697.8 bits, E=5.6e-214
• PF13597: NRDD
  • amino acids 116 to 705 (590 residues), 635.7 bits, E=1.2e-194
• PF01228: Gly_radical
  • amino acids 582 to 686 (105 residues), 106.2 bits, E=1.9e-34

Best Hits

Swiss-Prot: 95% identical to NRDD_SALTY: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_09665)

MetaCyc: 94% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Sequence Analysis Tools

See A0A285AWU5 at UniProt or InterPro

Protein Sequence (712 amino acids)

>BWI76_RS02935 anaerobic ribonucleoside triphosphate reductase (Klebsiella michiganensis M5al)
MTPHVMKRDGCKVPFKSERINEAILRAAKAAGVDDADYCATVAEVVSQQMQGRAQVDISE
IQTAVENQLMSGPYKQLARAYIEYRHDRDSQREKRGRLNQEIRGLVEQTNSALLNENANK
DSKVIPTQRDLLAGIVAKHYARQHLLPHDVVMAHERGVIHYHDLDYSPFFPMFNCMLIDL
KGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVTESF
NKHRKIAEEWHIPDAEGYARSRTEKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTS
WESRLIQQSILRNRIAGLGKNRKTAVFPKLVFAIRDGLNHKFGDANYDIKQLALECASKR
MYPDILNYDQVVKVTGSFKTPMGCRSFLGVWENEDGEQVHDGRNNLGVISLNLPRIALEA
KGDETEFWKLLDERLQLARKALMTRIARLEGVKARVAPILYMEGACGVRLKADDDVSEIF
KNGRASISLGYIGIHETINALFGNKHMYDSAALREKGVAIVQRLREAVDQWKDETGYGFS
LYSTPSENLCDRFCRLDTAEFGVIEGVTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPL
ANGGFICYGEYPNIQHNLRALEDVWDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGF
TCPKCGNHDASRVSVTRRVCGYLGSPDARPFNAGKQEEVKRRVKHLGNGQIG