Protein Info for BWI76_RS01655 in Klebsiella michiganensis M5al

Annotation: homoserine O-succinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 96% identical to METAS_KLEP3: Homoserine O-succinyltransferase (metAS) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K00651, homoserine O-succinyltransferase [EC: 2.3.1.46] (inferred from 96% identity to kva:Kvar_4852)

MetaCyc: 94% identical to homoserine O-succinyltransferase (Escherichia coli K-12 substr. MG1655)
Homoserine O-succinyltransferase. [EC: 2.3.1.46]

Predicted SEED Role

"Homoserine O-succinyltransferase (EC 2.3.1.46)" in subsystem Methionine Biosynthesis (EC 2.3.1.46)

MetaCyc Pathways

aspartate superpathway (24/25 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
L-methionine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.46

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AW46 at UniProt or InterPro

Protein Sequence (309 amino acids)

>BWI76_RS01655 homoserine O-succinyltransferase (Klebsiella michiganensis M5al)
MPIRVQDELPAVNFLRNENVFVMTTTRATTQEIRPLKVLILNLMPKKIETENQFLRLLSN
SPLQLDIQLLRIDARESRNTPAEHLNNFYCNFEDICDQNFDGLIVTGAPLGLVEFNDVAY
WPQIKQVLEWAKDHVTSTLFVCWAVQAALNILYGIPKQTRSEKISGVYEHHILQPHALLT
RGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGDAYLFASKDKRIAFVTGHPEYDA
NTLASEFFRDVEAGLDPQVPYNYFPQNDPQNKPRATWRSHGNLLFINWLNYYVYQITPYD
LRHMNPTLD