Protein Info for BWI76_RS01295 in Klebsiella michiganensis M5al

Updated annotation (from data): Uridine phosphorylase (EC 2.4.2.3)
Rationale: Specifically important for utilizing Uridine. Automated validation from mutant phenotype: the predicted function (URPHOS-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: uridine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01718: uridine phosphorylase
  • amino acids 6 to 253 (248 residues), 431.3 bits, E=4.7e-134
• PF01048: PNP_UDP_1
  • amino acids 20 to 245 (226 residues), 133.3 bits, E=4.8e-43

Best Hits

Swiss-Prot: 98% identical to UDP_SALTI: Uridine phosphorylase (udp) from Salmonella typhi

KEGG orthology group: K00757, uridine phosphorylase [EC: 2.4.2.3] (inferred from 97% identity to eco:b3831)

MetaCyc: 97% identical to uridine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]

Predicted SEED Role

"Uridine phosphorylase (EC 2.4.2.3)" (EC 2.4.2.3)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• pyrimidine ribonucleosides degradation (2/2 steps found)
• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.2, 2.4.2.3

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3

Sequence Analysis Tools

See A0A285AW26 at UniProt or InterPro

Protein Sequence (253 amino acids)

>BWI76_RS01295 Uridine phosphorylase (EC 2.4.2.3) (Klebsiella michiganensis M5al)
MSKSDVFHLGLTKNDLQGATLAIVPGDPERVEKIAALMDKPVKLASHREFTTWRAELDGK
AVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHINVGDVLVTTASVRLDGAS
LHFAPMEFPAVADFECTTALVEAAKSIGATTHIGVTASSDTFYPGQERYDTFSGRVVSRF
KGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAGVIVNRTQQEIPNAETMKQTESH
AVKIVVEAARRLL