Protein Info for BWI76_RS01280 in Klebsiella michiganensis M5al
Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to METE_ESCF3: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 93% identity to ecv:APECO1_2647)MetaCyc: 93% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]
Predicted SEED Role
"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)
MetaCyc Pathways
- aspartate superpathway (24/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- L-methionine biosynthesis I (5/5 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
- S-adenosyl-L-methionine salvage I (4/4 steps found)
- S-adenosyl-L-methionine salvage II (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- seleno-amino acid biosynthesis (plants) (3/5 steps found)
- L-methionine biosynthesis III (2/4 steps found)
- superpathway of seleno-compound metabolism (6/19 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.14
Use Curated BLAST to search for 2.1.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AW56 at UniProt or InterPro
Protein Sequence (753 amino acids)
>BWI76_RS01280 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (Klebsiella michiganensis M5al) MTIINHTLGFPRVGLRRELKKAQESYWAGNTGREELLAVGRELRARHWDQQKQAGVDLLP VGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN TNYHYMVPEFVKGQRFKLSWTQLLDEVDEALALGHKAKPVLLGPVTYLWLGKVKGEPFDR LSLLNDILPVYRQVLAELAKRGIEWVQIDEPALVLELPQAWLDAFKPAYEALNGEVKLLL TTYFEGIADNLDTITALPVQGLHVDLVHGQDNVAELHKRLPADWLLSAGLINGRNVWRAD LSEKYAQVKAIVGKRELWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCEELALLR DALNSGDTAAIAEWSAPIQARRHSTRVHNAAVEKRLAAITAQDSQRQSAYQVRAAAQRAR FKLPAWPTTTIGSFPQTTEIRGLRLDFKKGNLDANNYRTGIAEHIRQAIMEQERLGLDVL VHGEAERNDMVEYFGEHLDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPEAITVEWAKYA QSLTEKPVKGMLTGPVTILCWSFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQIDE PALREGLPLKRSDWDAYLAWGVEAFRINAAVAQDDTQIHTHMCYCEFNDIMDSIAALDAD VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAERL WVNPDCGLKTRGWPETLSALTNMVKAAQNLRQA