Protein Info for BWI76_RS00715 in Klebsiella michiganensis M5al

Annotation: two-component sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 165 to 186 (22 residues), see Phobius details PF16527: CpxA_peri" amino acids 80 to 152 (73 residues), 43.6 bits, see alignment E=7.6e-15 PF00672: HAMP" amino acids 181 to 233 (53 residues), 43.1 bits, see alignment 8.3e-15 PF00512: HisKA" amino acids 239 to 299 (61 residues), 50.7 bits, see alignment E=3e-17 PF02518: HATPase_c" amino acids 345 to 454 (110 residues), 89.3 bits, see alignment E=4.7e-29

Best Hits

Swiss-Prot: 93% identical to CPXA_ECOLI: Sensor histidine kinase CpxA (cpxA) from Escherichia coli (strain K12)

KEGG orthology group: K07640, two-component system, OmpR family, sensor histidine kinase CpxA [EC: 2.7.13.3] (inferred from 98% identity to kpn:KPN_04219)

MetaCyc: 93% identical to sensor histidine kinase CpxA (Escherichia coli K-12 substr. MG1655)
Protein-histidine tele-kinase. [EC: 2.7.13.2]

Predicted SEED Role

"Copper sensory histidine kinase CpxA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.2 or 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVR5 at UniProt or InterPro

Protein Sequence (457 amino acids)

>BWI76_RS00715 two-component sensor histidine kinase (Klebsiella michiganensis M5al)
MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLESEQRQGIMIEQHVEAELAND
PPNDLMWWRRLFRAIDKWAPPGQRLLLVTSEGRVIGAERNEMQIIRNFIGQADNADHPQK
KRYGRLEMVGPFSVRDGEDNYQLYLIRPANTSQSDFINLLFDRPLLLLIVTMLVSAPLLL
WLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEFLAAGTSFNQMVTALERMMSSQH
RLLSDISHELRTPLTRLQLGTALLRRRSGESKELERIEMEAHRLDSMINDLLVMSRNQAK
TALVSETVKANQLWGEVLDNAAFEAEQMGKSFTVEYPPGPWPLYGNPNALESALENIVRN
ALRYSHTKISVSFSVDKDGITVNVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLG
LAIVEAAIQQHRGWVKADDSPLGGLRLTLWLPLYKRT