Protein Info for BWI76_RS00670 in Klebsiella michiganensis M5al

Updated annotation (from data): Rhamnulose-1-phosphate aldolase (EC 4.1.2.19)
Rationale: Specifically important for utilizing L-Rhamnose monohydrate. Automated validation from mutant phenotype: the predicted function (4.1.2.19) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: rhamnulose-1-phosphate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 TIGR02624: rhamnulose-1-phosphate aldolase" amino acids 1 to 272 (272 residues), 453 bits, see alignment E=1.6e-140 PF00596: Aldolase_II" amino acids 13 to 238 (226 residues), 83.3 bits, see alignment E=1e-27

Best Hits

Swiss-Prot: 97% identical to RHAD_KLEP7: Rhamnulose-1-phosphate aldolase (rhaD) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01629, rhamnulose-1-phosphate aldolase [EC: 4.1.2.19] (inferred from 96% identity to kpu:KP1_0070)

MetaCyc: 88% identical to rhamnulose-1-phosphate aldolase (Escherichia coli K-12 substr. MG1655)
Rhamnulose-1-phosphate aldolase. [EC: 4.1.2.19]

Predicted SEED Role

"Rhamnulose-1-phosphate aldolase (EC 4.1.2.19)" in subsystem L-rhamnose utilization (EC 4.1.2.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVQ5 at UniProt or InterPro

Protein Sequence (276 amino acids)

>BWI76_RS00670 Rhamnulose-1-phosphate aldolase (EC 4.1.2.19) (Klebsiella michiganensis M5al)
MQTIIDAWFVQGMIKATSDAWLKGWDERNGGNLTLRLDEADIEPYAADFHAKPRYIALSQ
PMPTLANQPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNDAVPTSELPA
HFLSHSERIKLTNGKDRVIMHCHATNLIALTYVLENSSDLFTRKLWEGSTECLVVFPDGV
GILPWMVPGTDEIGQATAEAMQKHSLVLWPFHGVFGSGPNLDETFGLIDTAEKSAEVLVK
VYSMGGMKQTITREELIALGKRFNVHPLQSALDLYQ