Protein Info for BWI76_RS00355 in Klebsiella michiganensis M5al

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 929 transmembrane" amino acids 760 to 779 (20 residues), see Phobius details TIGR00593: DNA polymerase I" amino acids 9 to 929 (921 residues), 949.9 bits, see alignment E=9.1e-290 PF02739: 5_3_exonuc_N" amino acids 9 to 170 (162 residues), 195.8 bits, see alignment E=8.5e-62 PF01367: 5_3_exonuc" amino acids 171 to 270 (100 residues), 104.2 bits, see alignment E=9.1e-34 PF01612: DNA_pol_A_exo1" amino acids 331 to 518 (188 residues), 138.8 bits, see alignment E=3.4e-44 PF00476: DNA_pol_A" amino acids 553 to 926 (374 residues), 561 bits, see alignment E=2.5e-172

Best Hits

Swiss-Prot: 91% identical to DPO1_SALTY: DNA polymerase I (polA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 90% identity to cko:CKO_03151)

MetaCyc: 90% identical to DNA polymerase I (Escherichia coli K-12 substr. MG1655)
3.1.11.-; 3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVG3 at UniProt or InterPro

Protein Sequence (929 amino acids)

>BWI76_RS00355 DNA polymerase I (Klebsiella michiganensis M5al)
MVQIPENPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLILQYQPTHAVV
VFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAMGLPLMAVPGVEADDVIGT
LAREAEKVGRPVLISTGDKDMAQLVTPGITLINTMTNTILGPDEVVTKYGVPPELIIDFL
ALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEPEKIAGLTFRGAKTMAAKLEQNK
EVAYLSYQLATIKTDVELDLGCEDLLVAQPIADELLTLFKKYEFKRWITDVESGKWMQAK
GAKPAAKPIAAVAAVADAEEETATALSSENYVTILDEETLLTWIESLKKAPLFAFDTETD
SLDNVSANMVGLSFAIEPGVAAYVPVAHDYLDAPDQIPRDRVLELLKPLLEDDNVLKVGQ
NLKYDRGILANYDIELRGIAFDTMLESYILDSVAGRHDMDSLSDRWLKHKTITFEEIAGK
GKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPKLQQNEGPLNVFKNIEMPLVPVLSR
IERNGVKIDPAVLHVHSQEIAKRLVELEKKAYDIAGEEFNLSSPKQLQTILFEKQGIKPL
KKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDKLPLMISPKTGRVHTSY
HQAVTATGRLSSTDPNLQNIPVRNDEGRRIRQAFIAPEDYLIVSADYSQIELRIMAHLSR
DKGLLTAFAEGKDIHRATAAEVFGLPLESVSSEQRRSAKAINFGLIYGMSAFGLARQLNI
PRKEAQKYMDLYFERYPGVLEYMERTRTQAKEQGYVETLDGRRLYLPDIKSSNGARRAGA
ERAAINAPMQGTAADIIKRAMIAVDSWLLSEKPRVRMIMQVHDELVFEVHKDDLEAVAKK
IHELMESSTTLAVPLLVEVGSGENWDQAH