Protein Info for BPHYT_RS33975 in Burkholderia phytofirmans PsJN

Annotation: 2-methylcitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 TIGR02330: 2-methylcitrate dehydratase" amino acids 13 to 483 (471 residues), 876 bits, see alignment E=3e-268 PF03972: MmgE_PrpD_N" amino acids 18 to 270 (253 residues), 314.3 bits, see alignment E=4.8e-98 PF19305: MmgE_PrpD_C" amino acids 287 to 465 (179 residues), 206.9 bits, see alignment E=2.1e-65

Best Hits

Swiss-Prot: 78% identical to PRPD_ECOLI: 2-methylcitrate dehydratase (prpD) from Escherichia coli (strain K12)

KEGG orthology group: K01720, 2-methylcitrate dehydratase [EC: 4.2.1.79] (inferred from 100% identity to bpy:Bphyt_6862)

MetaCyc: 78% identical to 2-methylcitrate dehydratase (Escherichia coli K-12 substr. MG1655)
2-methylcitrate dehydratase. [EC: 4.2.1.79]

Predicted SEED Role

"2-methylcitrate dehydratase (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.79

Use Curated BLAST to search for 4.2.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9Q2 at UniProt or InterPro

Protein Sequence (483 amino acids)

>BPHYT_RS33975 2-methylcitrate dehydratase (Burkholderia phytofirmans PsJN)
MSAPISNVRPDPDSVLADIVDYVRDYRIDSALALETARHCLIDTLGCGLEALTYPACTKL
MGPIVPGTIVPNGAKVPGTSFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
LATADWLSRSAIAAGKAPLAMKDVLIAMIKAHEIQGCIALENSFNKVGLDHVLLVKLAST
AVVGQLLDLTRDELINAVSQAFVDGHALRTYRHAPNTGSRKSWAAGDATSRAVRLALISK
TGEMGYPSVLTAKTWGFYDVLFKGNAFQFQRPYGSYVMENVLFKISFPAEFHAQTAVEAT
MKLHAQLNAAGRSVDDISKITIRTHEAAIRIIDKKGPLNNPADRDHCIQYMVAVPLIHGR
LTAADYEDSIAQDPRIDVLRAKMECVEDPQFTQDYHDPEKRSIANALTIEFNDGSSFDEV
IVEYPIGHKRRREDGIPLLVEKFKTNLARRFPVKQQLAILDVSLDQARLEAMPVNEYVDL
YVI