Protein Info for BPHYT_RS33610 in Burkholderia phytofirmans PsJN

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 913 transmembrane" amino acids 745 to 764 (20 residues), see Phobius details PF02739: 5_3_exonuc_N" amino acids 11 to 173 (163 residues), 192 bits, see alignment E=1.2e-60 TIGR00593: DNA polymerase I" amino acids 12 to 913 (902 residues), 904.4 bits, see alignment E=5e-276 PF01367: 5_3_exonuc" amino acids 174 to 257 (84 residues), 87.9 bits, see alignment E=1.1e-28 PF01612: DNA_pol_A_exo1" amino acids 316 to 501 (186 residues), 104.8 bits, see alignment E=9.2e-34 PF00476: DNA_pol_A" amino acids 535 to 911 (377 residues), 528.5 bits, see alignment E=1.9e-162

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 81% identity to bcj:BCAM0904)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9I4 at UniProt or InterPro

Protein Sequence (913 amino acids)

>BPHYT_RS33610 DNA polymerase I (Burkholderia phytofirmans PsJN)
MPEERSLEGKTLLLVDGSSYLYRAYHAMPDLRSPDGNPTGALYGMINMLRRMRKEVTAEY
SACVFDAKGKTFRDDWYPDYKANRPSMPDDLSRQIEPIHVAVRALGWPLLMIDGVEADDV
IGTLSTQAEKRGMNVIVSTGDKDLAQLVSDHVTLINTMTNETLDRAGVLAKFGVPPERIV
DYLSLIGDTVDNVPGVEKCGPKTAIKWLTQFDSLDGIVAHADEIKGAVGDNLRRALDFLP
MAKKLVTVERNCDMTDHIVSIEESLQSRPESREELREVFTRHGFKTWLREVEIADAVEGP
ETDVPPALSVDHERHYDTVQTWEQFDGWLEKINTAELTSFDTETTSLDPMTAQIVGLSLS
VEAGRAAYVPLAHRGPDAPVQLPRDEVLARLKPWLESAEHKKVGQHLKYDEQVLANYGIE
MRGIEHDTLLQSYVVESHRTHDMDSLALRHLGVKTIKYEEVAGKGAGQIGFDEVALDKAA
EYAAEDADITLRLHQALYPQVTAEKTLEYVYRAIELPTSRVLRKMERTGVLIDAEKLRLQ
SSEIATRLIQLESEAYVLAGGEFNLGSPKQIGQIFFEKLELPVVKKTPSGAPSTDEEVLQ
KLAEDYPLPKILLEHRGLSKLKSTYTDKLPRMVNATTGRVHTNYAQAVAVTGRLASNDPN
LQNIPVRTGEGRRIREAFIAPPGHKLVSADYSQIELRIMAHISGDESLLRAFSQGEDIHR
ATAAEIFSVTPLEVSNDQRRVAKVINFGLIYGMSSFGLAANLGITRDAAKLYIDRYFARY
PGVARYMDETRVSAKAKGYVETVFGRRLWLPEINGGNGPRRQAAERAAINAPMQGTAADL
IKLSMIAVQNWIEESKIGTRMIMQVHDELILEVPDAELSDVRKRLPELMCGVADLKVPLV
AEVGAGLNWEEAH