Protein Info for BPHYT_RS32805 in Burkholderia phytofirmans PsJN

Annotation: acylaldehyde oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 756 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF20256: MoCoBD_2" amino acids 46 to 105 (60 residues), 34.8 bits, see alignment 1.4e-12 amino acids 629 to 708 (80 residues), 48.6 bits, see alignment E=8.2e-17 PF02738: MoCoBD_1" amino acids 346 to 581 (236 residues), 126.6 bits, see alignment E=9.1e-41

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 100% identity to bpy:Bphyt_6629)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T925 at UniProt or InterPro

Protein Sequence (756 amino acids)

>BPHYT_RS32805 acylaldehyde oxidase (Burkholderia phytofirmans PsJN)
MLKRRTFLLGGAGAVGALVVGWSVLPPGQRLTTSMPLPAQGSQVALNGWVKIAADNSVTI
MMCKAEMGQGIHTGLAMLLAEELDADWSQVRVEDSPIDKIYNSVQSIIDDLPFRPDDDST
VKRTTVWMTRKLVREAGTMMTGGSSSINDLWTPMREAGASARAMLIGAAAAQWKVPPGEC
RAEGGQVLHPSGHKASFGELSSMAAQQALPRKVTLKDPANFKLIGKPLRRIEAASKINGT
ARFGIDVLPDGLLYASVEMCPTLGGSVAKFDATAAGKMPGFIKAFAVAPYNGGTGGVAVI
ADNPFRAMNAVAAVDVTWHDGPAAKLSNADADRRLAQALDDNDGHAYYHLGDVDAALTRA
ARTVSAEYRAPYLAHGAVEPLNCTVQVADGAATVWVSTQMPGLARHHVAKVLDLDADKVD
VQTFLLGGAFGRRLELDFIAQAAAIAREGGGRPVQTLWSRAQDFTHDFYRPACLSRLKAG
FDNDGKLVAWQAASASQAIVPEALARYYGAPRLPIDKTTCEGAFDQPYEWPAARVAHKIV
ELPVPVGFWRSVGHSHQAFFTEGFTDELAVATGKDPIAFRAALLEQHPRHLAVLKRAAAL
SDWEHPLTHAADGAPRARGVALHEAFGSVVAQVAEVSIGPGKKIRVHRVVCVIDCGIPVN
PNLIRQQMESAVVFGLSAALQDEITLVNGQVQQKNFLDFPVVRIDDCPVIDTDIMPSQLH
PQGVGEAGVPPVAPAVANAVFKLTGQRLRSLPLKLA