Protein Info for BPHYT_RS32460 in Burkholderia phytofirmans PsJN

Annotation: tRNA 2-selenouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 PF00581: Rhodanese" amino acids 11 to 125 (115 residues), 38.9 bits, see alignment E=5.2e-14 TIGR03167: tRNA 2-selenouridine synthase" amino acids 14 to 317 (304 residues), 353.9 bits, see alignment E=4.3e-110

Best Hits

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 100% identity to bpy:Bphyt_6564)

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBC1 at UniProt or InterPro

Protein Sequence (355 amino acids)

>BPHYT_RS32460 tRNA 2-selenouridine synthase (Burkholderia phytofirmans PsJN)
MKNLLVSLEHAGDFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTTYKQVSPFEA
TRMGAALVARNIAHHLETTFSDRPRNWRPLIYCWRGGKRSGSVTMLFNMIGWQARQLEGG
YKTYRRATLDTLVTLPKAFSYIALVGPTGSGKTRLLAALNTAGAQTLDLEALAAHRGSLL
GAWAGVPQPSQKRFDTLLVSALRTFDPARPVFVEAESRRIGSIALPLALLDTFHQGRCVE
VISNPEDRAAFLLHDYAHLFDDPELLKGQLQRMIGLHSRERITGWQQLVDDNRRADLAHE
LIERHYDPAYARSSRQHFVHLARALQWNFRPNDADVVDQAKALLAELDSSALAVV