Protein Info for BPHYT_RS30525 in Burkholderia phytofirmans PsJN

Annotation: nitrate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details PF12974: Phosphonate-bd" amino acids 83 to 174 (92 residues), 32.2 bits, see alignment E=1.5e-11 PF09084: NMT1" amino acids 89 to 201 (113 residues), 27.5 bits, see alignment E=5.8e-10 PF13379: NMT1_2" amino acids 144 to 241 (98 residues), 25.3 bits, see alignment E=2.6e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_6157)

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T8C2 at UniProt or InterPro

Protein Sequence (331 amino acids)

>BPHYT_RS30525 nitrate ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MAGSISTSFSARRRALKLLSAVPACAVAGAFNSLGVQAAELAPVTLVLGDQAGGTRALAE
AAKVLDGAPYAFRWANFQGAAPLFEAQRAGAVDLAPAGDLPVLAAAVGDPTLKIVATRVG
SGEQLGILVAQDSKIRTVADLKGRTVFVSSARGSISQFQLYGALAEAGLSKEDVNVRFIL
PVDAFAAFASGSIEVWATFDPYYGIAVQRGARILRDGTGINSGLGFITASGAALAEPRKR
AAIADVLLRFQRAGDWALAHPDAYAQIYASLTRSPLDAAKRITQRASLKQHFINEADISA
LQKVADSANRDAILPRRIDVRAISDTRIATA