Protein Info for BPHYT_RS29700 in Burkholderia phytofirmans PsJN

Annotation: cytochrome C1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details PF10605: 3HBOH" amino acids 7 to 685 (679 residues), 1079 bits, see alignment E=0

Best Hits

Swiss-Prot: 91% identical to HBOH_PARXL: D-(-)-3-hydroxybutyrate oligomer hydrolase (Bxeno_B0795) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K07518, hydroxybutyrate-dimer hydrolase [EC: 3.1.1.22] (inferred from 100% identity to bpy:Bphyt_5988)

Predicted SEED Role

"D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.22)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T853 at UniProt or InterPro

Protein Sequence (685 amino acids)

>BPHYT_RS29700 cytochrome C1 (Burkholderia phytofirmans PsJN)
MLAMSVVLAACHGGNDDNSPPQANTLPGFITASSVKMQAYDDNTDDLLTAGLGKTGLGSA
TAPAIANAAQPTAAELRRLAIWSNYRALVDMTANGGYGRFWGPNVDLNGNDTLGEGKIAG
KEYLAYADDGSGTQNVTMLVQIPASFNPDQPCIVTATSSGSRGVYGAISAAGEWGLKRGC
AVAYTDKGSGNGAHELSTNRVTLIDGTLQDAAAAGKASLFTANLANTDLATYNSQFPNRY
AFKHAHSQQNPEKDWGNDTLEAIQFAYWALNDSYGALNGSTRTVRYAGGSITTIAASVSN
GGGASLAAAEQDTSGWITAVVVGEPQINLRLPAQVSVAQGGVPVGAFGKPLADYMTLANQ
LQPCAALAPAAAGAPYLTALPLATTTAIRTARCASLASAGVIAGGNVTTQAINALALLHQ
NGYQTDSDLLQAPMWDSQAVPAVAVTYANAYAKASVTANLCDFSFGTTSATGTAGVTPAV
SPMLTVFGNGNGVPPTNGINLVFNSGGGAADHRLATPDGSYAGASCLRTLWTNGALTNSV
EAIRVNANLHGKPAIIVQGRADALVPINHASRPYLGLNQLSEGGASKLSFYEVTNAQHFD
AFLGVAGFDTRFVPLHYYNIQALNLMWSHLKSGTPLPPSQVIHTVPRGGTAGAAPALTTA
NLPAIAASPGINAITVTNGAVNVPN