Protein Info for BPHYT_RS28220 in Burkholderia phytofirmans PsJN

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 PF04909: Amidohydro_2" amino acids 4 to 297 (294 residues), 136.9 bits, see alignment E=5.9e-44

Best Hits

Swiss-Prot: 46% identical to Y4MH_SINFN: Uncharacterized protein y4mH (NGR_a02510) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K07046, (no description) (inferred from 100% identity to bpy:Bphyt_5685)

Predicted SEED Role

"L-fuconolactone hydrolase" in subsystem L-fucose utilization temp

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TFM3 at UniProt or InterPro

Protein Sequence (297 amino acids)

>BPHYT_RS28220 amidohydrolase (Burkholderia phytofirmans PsJN)
MQVVDSHIHLWDLKTHRYPWLENPGVSFVGDARDLKHDYLLDDLLGEAGDIDVLKLVHVE
ANHDPAAPVEETRWLQAIADRKESCGMPNAIVAAVDLSAPNAPAVLEAHASFANTRGVRQ
ILNVHENKLFDYVGRHLMRERQWREHFALLRRYGMSFDLQLYPSQMEEAAALARAHGDTQ
FVINHAGMFVDRGSVAGYRAWREGMRLLADCPNIAVKISGLAMFDHRWTVESLRPYVLET
IDTFGVERAMFASNFPVDRLFGSYADLWHAYASIVEVASVAEKEALFCRNAERCYRI