Protein Info for BPHYT_RS27435 in Burkholderia phytofirmans PsJN

Annotation: amylo-alpha-1,6-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 757 PF14742: GDE_N_bis" amino acids 41 to 231 (191 residues), 194.2 bits, see alignment E=3e-61 PF06202: GDE_C" amino acids 328 to 684 (357 residues), 36.7 bits, see alignment E=3.8e-13 PF17389: Bac_rhamnosid6H" amino acids 412 to 546 (135 residues), 25.8 bits, see alignment E=9.4e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5524)

Predicted SEED Role

"Glycogen debranching enzyme"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TG05 at UniProt or InterPro

Protein Sequence (757 amino acids)

>BPHYT_RS27435 amylo-alpha-1,6-glucosidase (Burkholderia phytofirmans PsJN)
MQQPEALGGLSGGIDHREPEPDGAFIPTENLNVASATQHVLKSGDTFIVNDPLGDITGHD
DGLFVNDTRVLSQLRLTFGGRAPSLLSGSVSSDNTSFTAHLTNRPLPPLGGDSTPEGVIH
VERVRVLSGTVLNEAIELTNYGTSDAVVPLSISFASDFRDMFEVRGLKREKQGRVEPARV
ENREVLLGYVGLDDVARNVRIAFSPEPDKLFADRADYTVKLPAQACVSIYLSVSVQVVAQ
TDKPIAGVSQPTHALSEAHLSQIDAERPRVGRAAVRAALVDAHLVMRERRRVTARVRSSN
PLFNAWIDRSLADLGLLTTDLVTGPYPYAGIPWFSTPFGRDAVITSLQTLWLQPQLAAGV
LRFLAEHQARENSPFRDAAPGKIMHEMRKGEMAATGEVPFALYYGGVDTTPLFIVLAGAY
AARTSDLALIDELWPALERAAQWVAGVCDKNRYGLLDYQRESDGGLANQGWKDSHDSVFH
ADGRFPDGPIALVEVQAYASAAFDTMAHFAKLRGLHDAAEQYSERAKKIRQCVEEKYWME
EAGFYGIALDGHGDLCRVMASNAGHLLAFGLPSRERGESVVRALDSTLFHTGWGVRTLAA
SQSRFNPMAYHNGSVWPHDNALCARGLSRYGGKAAAVRLLQALFQAAVNFDMRLPELFCG
FPRRRGEPPTAYPVACLPQAWAAGSPFMMLEACLGITIDAERREVLIEQPMLPEGIDWLE
VGDLRVGDASVSITFRRIGDKVVASAEQGDVRVIALL