Protein Info for BPHYT_RS26965 in Burkholderia phytofirmans PsJN

Updated annotation (from data): L-lactate dehydrogenase, LldG subunit
Rationale: Specifically important for utilization of L-lactate and D,L-lactate as well as L-rhamnose and L-fucose, which are catabolized via L-lactate. This gene has additional phenotypes which were not found for the other subunits and are not explained.
Original annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 195 PF02589: LUD_dom" amino acids 94 to 190 (97 residues), 41 bits, see alignment E=9.2e-15

Best Hits

KEGG orthology group: K00782, hypothetical protein (inferred from 100% identity to bpy:Bphyt_5427)

Predicted SEED Role

"Predicted L-lactate dehydrogenase, hypothetical protein subunit SO1518" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TBY7 at UniProt or InterPro

Protein Sequence (195 amino acids)

>BPHYT_RS26965 L-lactate dehydrogenase, LldG subunit (Burkholderia phytofirmans PsJN)
MATREAFLDKVRQAQPPSRPRPEVPLFDAPGGDLRVRFTAALQAMGGTCVQAVSATDVSG
LIRERFGAEASVASATTEVPGTRALTADTEPASLQDIDVGVVRARFGVAETGSVWFSERE
YVVNALGYIVQHLIVLLDPAQLIDGLQDVYRRDDFRDARYAALVTGPSATADIEGVLIRG
AQGVRSLTVVWLAAQ