Protein Info for BPHYT_RS25045 in Burkholderia phytofirmans PsJN

Annotation: LysR family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00126: HTH_1" amino acids 8 to 66 (59 residues), 66.1 bits, see alignment E=2.2e-22 PF03466: LysR_substrate" amino acids 92 to 300 (209 residues), 137.9 bits, see alignment E=3.2e-44

Best Hits

Swiss-Prot: 35% identical to TTDR_ECOL5: HTH-type transcriptional activator TtdR (ttdR) from Escherichia coli O6:K15:H31 (strain 536 / UPEC)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_5036)

Predicted SEED Role

"Positive regulator of Tartrate dehydrogenase/decarboxylase/D-malic enzyme" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCH6 at UniProt or InterPro

Protein Sequence (303 amino acids)

>BPHYT_RS25045 LysR family transcriptional regulator (Burkholderia phytofirmans PsJN)
MTSSIQPDDLAFFSTLAASGSLTAAARELGLSTAAVSKHLAQMESRAAVSLVNRTTRRMS
LTPEGELYLTRARRILSEMDDLAQLLGGSQAAPKGLLRVNATLGFGRSHVAPLISRFVRR
FPEVDVQLQLSVDPPPLSDDAFDVCVRFGEPPDARVIARRVASNRRLLCASPRYLAERRA
PRSPAELAQHNCIGIRQGGDAYGVWRLTSGRGAAEKTETVRVRGNLTTNDGEIAVKWALD
GQGILMRAEWDIAEYLADGRLVHVLPEYRTPGADIYAVYPQQLQMTARVKAFVDMLVETL
GVR