Protein Info for BPHYT_RS24700 in Burkholderia phytofirmans PsJN

Annotation: 3-methyladenine DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 TIGR00567: DNA-3-methyladenine glycosylase" amino acids 18 to 196 (179 residues), 136.5 bits, see alignment E=4e-44 PF02245: Pur_DNA_glyco" amino acids 19 to 192 (174 residues), 164.7 bits, see alignment E=7.5e-53

Best Hits

Swiss-Prot: 100% identical to 3MGH_PARPJ: Putative 3-methyladenine DNA glycosylase (Bphyt_4972) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K03652, DNA-3-methyladenine glycosylase [EC: 3.2.2.21] (inferred from 100% identity to bpy:Bphyt_4972)

Predicted SEED Role

"DNA-3-methyladenine glycosylase II (EC 3.2.2.21)" in subsystem DNA Repair Base Excision (EC 3.2.2.21)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.21

Use Curated BLAST to search for 3.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCB2 at UniProt or InterPro

Protein Sequence (213 amino acids)

>BPHYT_RS24700 3-methyladenine DNA glycosylase (Burkholderia phytofirmans PsJN)
MRKHQLPILPLLRDDLPLDTVELARFMIGKYLVHDLPEGRMSGRIVETEAYPLGDSTSHA
FMGRRPHNGSMFLAPGHAYVRLTYGLSYMLNMSAEAEEVGAGILLRAIEPLEGLPLIEAR
RPGVPLRDLARGPGRLTMAFGVGPSFDGWDLCSGQGLWIGVIERGEVPVGVTTRIGLSRE
MHQPLRFFEPGSAFVSGPRKLLVTPQSGAPKRA