Protein Info for BPHYT_RS24500 in Burkholderia phytofirmans PsJN

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 transmembrane" amino acids 14 to 33 (20 residues), see Phobius details amino acids 172 to 191 (20 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 7 to 464 (458 residues), 452.7 bits, see alignment E=8.3e-140 PF00672: HAMP" amino acids 190 to 241 (52 residues), 42.1 bits, see alignment 1.4e-14 PF00512: HisKA" amino acids 247 to 311 (65 residues), 49.6 bits, see alignment E=5.1e-17 PF02518: HATPase_c" amino acids 358 to 464 (107 residues), 70.4 bits, see alignment E=2.5e-23

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to bpy:Bphyt_4934)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TC78 at UniProt or InterPro

Protein Sequence (466 amino acids)

>BPHYT_RS24500 histidine kinase (Burkholderia phytofirmans PsJN)
MTFWTGRSLTARTTVLFASIACVVIGTLGAYFYHSAEVSLQRRADVVLTARVEHFSRIVR
DLYSVGELKSRPLLFETMLGAEQDVLLFRRPGEAPFIDVNPAGVAVPALQAGVEGRLPTF
ADVRQTRLANGVPVHWAIATAKAREDGTEVEVIAGHPMTEEVRMLAAYRDRIVLATLIGM
LAATLLAWYVLRRALRPVREIATRAAQISPTSLSVRLDSEAAPLELRQLTHAFNAMLERL
ADGYQRLSQFSADLAHEIRTPVGALIGQTQVTLARIREPDEYQQVLESNLEELSRLSHIA
ENILFLAHADHAALSVEREPVDLREELVRIADYFEGPADERGMRFVVEATGVASANPMLC
RRAINNLVVNAVRYGASDTVVRLRGTQDEAGATVVVENDGAPIPEEQLGRLFDRFYRADT
ARSAFTESSGLGLAIVKAIMHLHGGTARVVCPAPGVVRFELRFPGA