Protein Info for BPHYT_RS23135 in Burkholderia phytofirmans PsJN

Annotation: putrescine/spermidine ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 64 to 88 (25 residues), see Phobius details amino acids 99 to 125 (27 residues), see Phobius details amino acids 137 to 157 (21 residues), see Phobius details amino acids 181 to 202 (22 residues), see Phobius details amino acids 240 to 261 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 80 to 267 (188 residues), 53.5 bits, see alignment E=1.3e-18

Best Hits

Swiss-Prot: 58% identical to POTI_ECOL6: Putrescine transport system permease protein PotI (potI) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11074, putrescine transport system permease protein (inferred from 100% identity to bpy:Bphyt_4661)

MetaCyc: 58% identical to putrescine ABC transporter membrane subunit PotI (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotI (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDJ1 at UniProt or InterPro

Protein Sequence (303 amino acids)

>BPHYT_RS23135 putrescine/spermidine ABC transporter permease (Burkholderia phytofirmans PsJN)
MIKPNKTLSNSVLTFGFLFLYIPIISLIVYSFNESKLVTVWSGFSLKWYGALLQDGELLN
AAWLSLKIGLLTACASVVIGTWAGFVLARFGRFRGFTLFAGMINAPLVIPEVIQGISLLL
LFVALEQMLGWPKGRGLFTIWIGHVMLCVSYVAIIVQSRVKELNRSLEEAALDLGATPFK
VFFLITLPLISQALMSGWLLSFTLSFDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATIFITTVTIGVIAVNRWMQLRERKRNRDMQMAFALAEAADPLPAAPQQAAARKSLDT
ARA