Protein Info for BPHYT_RS22100 in Burkholderia phytofirmans PsJN

Annotation: ribonuclease BN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 transmembrane" amino acids 41 to 64 (24 residues), see Phobius details amino acids 104 to 129 (26 residues), see Phobius details amino acids 151 to 176 (26 residues), see Phobius details amino acids 189 to 213 (25 residues), see Phobius details amino acids 224 to 246 (23 residues), see Phobius details amino acids 256 to 279 (24 residues), see Phobius details amino acids 417 to 434 (18 residues), see Phobius details TIGR00765: YihY family inner membrane protein" amino acids 22 to 283 (262 residues), 93.3 bits, see alignment E=1.2e-30 PF03631: Virul_fac_BrkB" amino acids 31 to 285 (255 residues), 172.6 bits, see alignment E=6.3e-55

Best Hits

KEGG orthology group: K07058, membrane protein (inferred from 100% identity to bpy:Bphyt_4448)

Predicted SEED Role

"Ribonuclease BN (EC 3.1.-.-)" in subsystem LMPTP YfkJ cluster or tRNA processing (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TE32 at UniProt or InterPro

Protein Sequence (443 amino acids)

>BPHYT_RS22100 ribonuclease BN (Burkholderia phytofirmans PsJN)
MDIDTLSAENLQQVARKQANWAIGAFKQFAENRCAAMAASIAFYAAFSLAPTLVMVIAVA
GWFFGAEAARGELFNHIHGLLGDQAAAGVQTIVENAHHSGSAGGIAAVISLVTLVIGASA
TFSSLNSALNIVWPNTGPRSSSVIALVRVRLISFGLVLGVAFLLIVSLVLDTVITFVGKW
LWGDSPYIVIGNLLQLGVGLLVLAFAFAGLLKFLPDARVRWRDAFVGGIVAAVLFSAGKK
LFALYLAHAGMATSFGAAGSLAVLLMWLYFSAAVLLLGAEFSAARGRMHDPRGGWGMQND
TPPGSRAKLASVLAASTVAADAVPHADAQANGRAASDDATRSPALRAAKAVALDGAAPPA
TFRARAENRPSARATALKVGRTVVLAEAQATRIAAVTLIEASRKAVAADRYVRRHPWTSV
LFAATAGLAAAMVTRRHTGNNER