Protein Info for BPHYT_RS21680 in Burkholderia phytofirmans PsJN

Annotation: amino acid permease-associated protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 46 to 65 (20 residues), see Phobius details amino acids 101 to 123 (23 residues), see Phobius details amino acids 132 to 154 (23 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 207 to 232 (26 residues), see Phobius details amino acids 251 to 270 (20 residues), see Phobius details amino acids 290 to 313 (24 residues), see Phobius details amino acids 341 to 360 (20 residues), see Phobius details amino acids 366 to 387 (22 residues), see Phobius details amino acids 409 to 431 (23 residues), see Phobius details amino acids 437 to 454 (18 residues), see Phobius details PF00324: AA_permease" amino acids 18 to 435 (418 residues), 385.3 bits, see alignment E=6e-119 PF03845: Spore_permease" amino acids 18 to 290 (273 residues), 27.1 bits, see alignment E=2.8e-10 PF13520: AA_permease_2" amino acids 18 to 449 (432 residues), 136.7 bits, see alignment E=1.7e-43

Best Hits

Swiss-Prot: 46% identical to GABP_ECOLI: GABA permease (gabP) from Escherichia coli (strain K12)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 100% identity to bpy:Bphyt_4364)

MetaCyc: 46% identical to 4-aminobutanoate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-384; TRANS-RXN-57

Predicted SEED Role

"Aromatic amino acid transport protein AroP" in subsystem Aromatic amino acid degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TF70 at UniProt or InterPro

Protein Sequence (476 amino acids)

>BPHYT_RS21680 amino acid permease-associated protein (Burkholderia phytofirmans PsJN)
MQRSTDTTELKAGLKQRHMTMIALGGVIGAGLFVGSGVVVQQAGPAAVLSFLITGALVVL
VMRMLGEMACAMPAVGSFYEYARLAFGGKRASGNLAGFLTGWMYWYFWVIVVAVEAVAGA
KLVQFWLPDVPAWAISLVLLVTLTATNLVSVGSYGEFEFWFASIKVAAIMVFLFLGGMYV
LGLWPAAKHVTAVLPTLLSHGGLMPKGIGPVLSGAVAATGFYFGAEIVTIAAAEAQEPAK
AVAKATNSVITRVLVFYVGSILLVVALVPWNSPKMATPYVSALDAMGIPAAASVMNAIVL
TAVLSALNSGLYAASRMIFALTRHGDAPAALAKVNRRGVPVRAILIGTVFGYASVVMSYV
SPDTVFAFLVNSYGTVAIFVYVLIAISQLKLRARIERDAPEKLRVRMWCYPYLTWVAIIG
MVGILVAMAFIPEQRQPLWFGVASLGVLLLAYLLRVRKPDAGFGEPDTLEYRHHHH