Protein Info for BPHYT_RS18595 in Burkholderia phytofirmans PsJN

Annotation: multifunctional CCA protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01743: PolyA_pol
  • amino acids 3 to 127 (125 residues), 57.3 bits, E=3.2e-19
• PF12627: PolyA_pol_RNAbd
  • amino acids 153 to 216 (64 residues), 47.1 bits, E=2.4e-16
• PF01966: HD
  • amino acids 234 to 313 (80 residues), 32.1 bits, E=1.9e-11

Best Hits

Swiss-Prot: 100% identical to CCA_PARPJ: Multifunctional CCA protein (cca) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 100% identity to bpy:Bphyt_3735)

MetaCyc: 55% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Ether lipid metabolism
• Fructose and mannose metabolism
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Riboflavin metabolism
• Sphingolipid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

See B2T6V1 at UniProt or InterPro

Protein Sequence (410 amino acids)

>BPHYT_RS18595 multifunctional CCA protein (Burkholderia phytofirmans PsJN)
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGKDFPVFLHPQTHEEYAL
ARTERKTAAGYHGFQFFYAPDVTLEEDLARRDLTINAMAREVRPDGELTGPVIDPFNGQG
DVQARVFRHVSDAFLEDPVRILRIARFAARFVDFTVAPETLALMRKMVADGEVDALVAER
VWQEVSRGLMEKKPSRMFEVLRECGALARILPEIDALFGVLQRADYHPEVDTGVHVMMVV
DHAAQQGYALQVRFAALAHDLGKATTPEDMLPRHIGHEGRSVDLLKPLCERLRVPNDCRD
LAVLVAREHGNIHRVMEMGAAALVRLLERSDAIRKPARFAEALQACEADARGRLGFERRE
YPQAERLRVALVAARGVDAGAVAKRLADAPAGIKDALHQERVRAVELAIS