Protein Info for BPHYT_RS18150 in Burkholderia phytofirmans PsJN

Annotation: DNA-directed RNA polymerase subunit beta'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1412 PF04997: RNA_pol_Rpb1_1" amino acids 17 to 342 (326 residues), 284.9 bits, see alignment E=2.2e-88 TIGR02386: DNA-directed RNA polymerase, beta' subunit" amino acids 19 to 1373 (1355 residues), 1808 bits, see alignment E=0 PF00623: RNA_pol_Rpb1_2" amino acids 344 to 396 (53 residues), 34.9 bits, see alignment (E = 4.7e-12) amino acids 391 to 485 (95 residues), 98.2 bits, see alignment 1.6e-31 PF04983: RNA_pol_Rpb1_3" amino acids 489 to 649 (161 residues), 82.8 bits, see alignment E=6.5e-27 PF05000: RNA_pol_Rpb1_4" amino acids 679 to 769 (91 residues), 76.5 bits, see alignment 3.7e-25 PF04998: RNA_pol_Rpb1_5" amino acids 771 to 1322 (552 residues), 265.6 bits, see alignment E=1.3e-82

Best Hits

Swiss-Prot: 78% identical to RPOC_ACIAC: DNA-directed RNA polymerase subunit beta' (rpoC) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K03046, DNA-directed RNA polymerase subunit beta' [EC: 2.7.7.6] (inferred from 66% identity to aat:D11S_0064)

Predicted SEED Role

"DNA-directed RNA polymerase beta' subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T758 at UniProt or InterPro

Protein Sequence (1412 amino acids)

>BPHYT_RS18150 DNA-directed RNA polymerase subunit beta' (Burkholderia phytofirmans PsJN)
MKALLDLFKQVQQPEVFDAIKIGLASPDKIRSWSFGEVKKPETINYRTFKPERDGLFCAK
IFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERMGHIELASPVAHIWFLKSL
PSRLGMVLDMTLRDIERVLYFEAYVVIDPGMTPLKARQIMTEEDYYNKVEEYGDEFRAEM
GAEGVRELLRAINIDEQVEMLRTELKNTGSEAKIKKYAKRLKVLEAFQRSGIKPDWMVLE
VLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLELKAPEIIVRNEKRMLQ
EAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGKGGRFRQNLLGKRVDYSGRSVIVVGPT
LKLHQCGLPKLMALELFKPFIFNKLEVMGVATTIKAAKKEVENQTPVVWDILEEVIREHP
VMLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSLEAQMEA
RTLMLASNNVLFPANGDPSIVPSQDIVLGLYYATREAVNAKGEGLTFTGVSEALRAYENK
EVELASRVNVRITEMVHNEDKSEGAPAFVPKITLYPTTVGRAILSEILPPGLPFSVLNKP
LKKKEISRLINTAFRKCGLRETVIFADQLMQSGFRLATRAGISICVDDMLVPPQKETIVG
DAAKKVKEYDRQYMSGLVTSQERYNNVVDIWSATSEAVGKAMMEQLSTEPVTDRDGNETR
QESFNSIYMMADSGARGSAVQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFIS
THGARKGLADTALKTANSGYLTRRLVDVTQDLVVVEDDCGTSNGVAMKALVEGGEVVEAL
RDRILGRVTVADVVNPESQETLYETGTLLDEDAVEEIERLGIDEVRVRTPLTCETRYGLC
AACYGRDLGRGSSVNVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSN
GTVRFTATMRYVTNAKGEQIVISRSGEAMITDDHGRERERHKVPYGATLLQLDGAQIKAG
TQLATWDPMTRPIITEWGGTVKFENVEEGVTVAKQIDDVTGLSTLVVIDVKRRGSQASKT
VRPQVKLLDANGEEVKIPNTEHSVQIGFQVGALITVKDGQQVQVGEVLARIPVESQKTRD
ITGGLPRVAELFEARSPKDAGILAEVTGTTSFGKDTKGKQRLVITDLDGNQHEFLIAKEK
QVLVHDGQVVNKGEMIVDGPADPHDILRLQGIEALSRYIVDEVQDVYRLQGVKINDKHIE
VIVRQMLRRVQITDNGDTRFIPGEQVERSDMLDENDRMIAEDKRPATYENVLLGITKASL
STDSFISAASFQETTRVLTEAAIMGKRDDLRGLKENVIVGRLIPAGTGLAFHKARKSKEL
SDRERFDQIAAEESFEFGTPETPAAEQQHSGE