Protein Info for BPHYT_RS18145 in Burkholderia phytofirmans PsJN

Annotation: ATP-dependent DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 6 to 613 (608 residues), 784 bits, see alignment E=8.8e-240 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 6 to 467 (462 residues), 494.6 bits, see alignment E=3e-152 PF00270: DEAD" amino acids 21 to 180 (160 residues), 82.1 bits, see alignment E=1.2e-26 PF00271: Helicase_C" amino acids 239 to 332 (94 residues), 61.1 bits, see alignment E=3.6e-20 PF16124: RecQ_Zn_bind" amino acids 344 to 405 (62 residues), 73.1 bits, see alignment E=7.7e-24 PF09382: RQC" amino acids 407 to 522 (116 residues), 124.3 bits, see alignment E=6.7e-40 PF00570: HRDC" amino acids 546 to 612 (67 residues), 83 bits, see alignment E=3.5e-27

Best Hits

Swiss-Prot: 47% identical to RECQ_SALTY: ATP-dependent DNA helicase RecQ (recQ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 100% identity to bpy:Bphyt_3650)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T757 at UniProt or InterPro

Protein Sequence (615 amino acids)

>BPHYT_RS18145 ATP-dependent DNA helicase RecQ (Burkholderia phytofirmans PsJN)
MSRPLEILNEVFGYPAFRGQQGEIVEHVAGGGDCLVLMPTGGGKSLCYQIPSLVRREGGF
GTGIVVSPLIALMQDQVAALTEVGVRAAYLNSTLSSAEAMATERALRDGDIDLLYVAPER
LMTPRFQELLERTRIGLFAIDEAHCVSQWGHDFRPEYIQLSVLHERFPNVPRIALTATAD
DITRDEIIHRLALDDARVFVSSFDRPNIRYRIVEKDNARAQLLDFIRAEHSKPDGTTDAG
VVYCLSRRKVEETAEWLKEKGMSALPYHAGMEFEIRQKHQEMFQREEGIVMCATIAFGMG
IDKPDVRFVAHLDLPKSVEGYYQETGRAGRDGMPANAWMAYGLGDVVQQRKMIDESDADD
AHKRVQTGKLDALLGLCEAATCRRVRLLGYFGESSKPCGNCDNCLEPPDTWDATREARMA
LSCVFRAQRASGFHFGAGHLIDILRGNSSEKILQRGHEKLTTFGIGSALGEQEWRAVFRQ
LVAFGYLTVDHEGFGSLVLTEASKPVLKGEQNVTMRRYVKPTRTRQSSGRTSERADPTIG
MGPRERARWERLRAWRTETAKSDGVPAYVIFHDATLAEIARNGPDSIDDLRGIPGMGARK
LDRFGDELLEVVAAD