Protein Info for BPHYT_RS17755 in Burkholderia phytofirmans PsJN

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 186 transmembrane" amino acids 7 to 27 (21 residues), see Phobius details TIGR04430: outer membrane lipid asymmetry maintenance protein MlaD" amino acids 5 to 148 (144 residues), 196.9 bits, see alignment E=7.2e-63 PF02470: MlaD" amino acids 39 to 117 (79 residues), 82.6 bits, see alignment E=9.8e-28

Best Hits

Swiss-Prot: 45% identical to MLAD_ECO57: Intermembrane phospholipid transport system binding protein MlaD (mlaD) from Escherichia coli O157:H7

KEGG orthology group: K02067, putative ABC transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_3569)

Predicted SEED Role

"Uncharacterized ABC transporter, periplasmic component YrbD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ70 at UniProt or InterPro

Protein Sequence (186 amino acids)

>BPHYT_RS17755 ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MKKTAALDFWVGLFVVLGFVALLFLALKAGNMSSLSFQPTYPVKLKFDNIGGLKARAPVK
SAGVTVGRVASIGFDSNAYQALVTIDLDKQYQFPKDTSAKILTSGLLGEQYIGLEPGGDS
EMLKAGDTISMTQSAIVLENLIGQFLYSKAADSGASKPGAAAGAPTPAPSAAPAASSLPA
SGAAAQ