Protein Info for BPHYT_RS17515 in Burkholderia phytofirmans PsJN

Annotation: peptide chain release factor 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 TIGR00019: peptide chain release factor 1" amino acids 1 to 355 (355 residues), 542.1 bits, see alignment E=2.7e-167 PF03462: PCRF" amino acids 13 to 203 (191 residues), 248 bits, see alignment E=7e-78 PF00472: RF-1" amino acids 214 to 321 (108 residues), 151.3 bits, see alignment E=1e-48

Best Hits

Swiss-Prot: 100% identical to RF1_PARPJ: Peptide chain release factor 1 (prfA) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K02835, peptide chain release factor 1 (inferred from 100% identity to bpy:Bphyt_3522)

Predicted SEED Role

"Peptide chain release factor 1" in subsystem LMPTP YwlE cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ27 at UniProt or InterPro

Protein Sequence (360 amino acids)

>BPHYT_RS17515 peptide chain release factor 1 (Burkholderia phytofirmans PsJN)
MKTSMQRKLDQLTTRLAELNDLLSREDITSNLDQYRKLTREHAELGPVVDHYALWRQAMN
DATTAQELLADASMRDFAEDEIRAARERMEKLGAELQKMLLPKDPNDDRNIFLEIRAGTG
GDESALFAGDLLRMYLRFAERNRWQVEMMSASESDLGGYKEVIVRIAGEAAYSKLKFESG
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQSHEQQAKEAATRKSLIGS
GDRSERIRTYNFPQGRLTDHRINLTLYRLDAIMDGDLDELIAALVSEHQAELLASLGDAD