Protein Info for BPHYT_RS17455 in Burkholderia phytofirmans PsJN

Annotation: glycerol-3-phosphate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01547: SBP_bac_1" amino acids 43 to 340 (298 residues), 111.5 bits, see alignment E=8.2e-36 PF13416: SBP_bac_8" amino acids 47 to 361 (315 residues), 124 bits, see alignment E=1.1e-39

Best Hits

Swiss-Prot: 70% identical to UGPB_YERE8: sn-glycerol-3-phosphate-binding periplasmic protein UgpB (ugpB) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K05813, sn-glycerol 3-phosphate transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_3509)

MetaCyc: 69% identical to sn-glycerol 3-phosphate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-34-RXN [EC: 7.6.2.10]; 7.6.2.10 [EC: 7.6.2.10]

Predicted SEED Role

"Glycerol-3-phosphate ABC transporter, periplasmic glycerol-3-phosphate-binding protein (TC 3.A.1.1.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization (TC 3.A.1.1.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ14 at UniProt or InterPro

Protein Sequence (441 amino acids)

>BPHYT_RS17455 glycerol-3-phosphate ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MSFKPLIRSLSLAAVLSVGLSQAAHAATEIQFWHGMEAALGERLNDIANAFNASQSDYKI
VPVFKGTYDQTLAAGIAAYRSGNAPAILQVYEVGTATMMQAKKAVIPVSEVFKQAGVPLD
EKAFVPTIASYYSDAKTGELISMPFNSSTPVLYYNKDAFKKAGLDPNQPPKTWAELQTDA
QKLKASGMACGYSSGWQSWIQLENYSAWHGAPFASKNNGFDGPDAQLEFNKPLQVAHIQF
LQNMQKEGTFTYVGRKDEPVSKFYSGDCGIITNSSGSLATIKKYAKFNFGTGMMPYDASV
KGAPQNAIIGGASLWVLSGKDPAVYKGVAKFLSYLSSAPVAAKWHQDTGYLPVTTAAYQL
TQQQGFYDKNPGSDTAIKQMLNKPPLPYTKGLRLGNMPQIRTVIDEELEQVWAQKKTPQQ
ALDASVSRGDELLRRFEKAGN