Protein Info for BPHYT_RS17415 in Burkholderia phytofirmans PsJN

Annotation: uracil-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 TIGR00628: uracil-DNA glycosylase" amino acids 56 to 265 (210 residues), 251.8 bits, see alignment E=3e-79 PF03167: UDG" amino acids 101 to 261 (161 residues), 75.4 bits, see alignment E=2.5e-25

Best Hits

Swiss-Prot: 65% identical to UNG_RALPJ: Uracil-DNA glycosylase (ung) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K03648, uracil-DNA glycosylase [EC: 3.2.2.-] (inferred from 94% identity to bxe:Bxe_A0456)

Predicted SEED Role

"Uracil-DNA glycosylase, family 1" in subsystem DNA Repair Base Excision

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ06 at UniProt or InterPro

Protein Sequence (281 amino acids)

>BPHYT_RS17415 uracil-DNA glycosylase (Burkholderia phytofirmans PsJN)
MTSASRTRSSSSQASLFGDATPTAAKAVMTTTQPQQSGASAPPPTLEAQFDALPPAWRTH
LKPFIESDAYAPLCRFVDGERAAGKTVYPADVFRALRLTSPDDVKVVILGQDPYHGEDRG
TPQAHGLAFSVAPNVRTPPSLRNIFKEIAASLGHETPRHGCLDTWAKQGVLLLNTVLTVE
RDSAASHAKRGWEKCTDTLIHELAMRHDGLVFMLWGAHAQAKRALLGGKSHCVLEAPHPS
PLSAHRGFLGCGHFAKANDYLAQHGREPIDWRLPDEAQMLA