Protein Info for BPHYT_RS16835 in Burkholderia phytofirmans PsJN

Annotation: glyceraldehyde-3-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 85% identical to G3PC_CUPNH: Glyceraldehyde-3-phosphate dehydrogenase, chromosomal (cbbGC) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 100% identity to bpy:Bphyt_3390)

MetaCyc: 58% identical to Gap (Thermotoga maritima)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). [EC: 1.2.1.12]

Predicted SEED Role

"NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Pyridoxin (Vitamin B6) Biosynthesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.12)

MetaCyc Pathways

superpathway of glucose and xylose degradation (16/17 steps found)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
Bifidobacterium shunt (14/15 steps found)
heterolactic fermentation (16/18 steps found)
superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
Entner-Doudoroff pathway I (9/9 steps found)
formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
glycolysis II (from fructose 6-phosphate) (10/11 steps found)
glycolysis III (from glucose) (10/11 steps found)
gluconeogenesis I (11/13 steps found)
glycolysis I (from glucose 6-phosphate) (11/13 steps found)
gluconeogenesis III (10/12 steps found)
homolactic fermentation (10/12 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
superpathway of anaerobic sucrose degradation (15/19 steps found)
superpathway of N-acetylneuraminate degradation (17/22 steps found)
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
glycolysis VI (from fructose) (8/11 steps found)
glycolysis IV (7/10 steps found)
glycerol degradation to butanol (11/16 steps found)
superpathway of hexitol degradation (bacteria) (12/18 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYP6 at UniProt or InterPro

Protein Sequence (336 amino acids)

>BPHYT_RS16835 glyceraldehyde-3-phosphate dehydrogenase (Burkholderia phytofirmans PsJN)
MTIRVAINGYGRIGRNTLRAFYENGKKHDIEIVAINDLGDAKTNAHLTQYDTAHGKFPGE
VSVDGDYLVVNGDKIRVLANRNPAELPWGELNVDVVLECTGFFTTKEKASAHIKGGAKKV
IISAPGGKDVDATIVYGVNHNVLKASDTVISNASCTTNCLAPLVKPLNDKIGLVNGLMTT
IHAYTNDQVLTDVYHEDLRRARSATHSQIPTKTGAASAVGLVLPELNGKLDGYAIRVPTI
NVSVVDLSFIAARDTTVEEVNAIMKEASEGSLKGILGYNEAPLVSIDFNHNPASSTFDAT
LTKVSGRLVKVSSWYDNEWGFSNRMLDTAVALANAK