Protein Info for BPHYT_RS16785 in Burkholderia phytofirmans PsJN

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 TIGR00396: leucine--tRNA ligase" amino acids 8 to 863 (856 residues), 1077.3 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 16 to 223 (208 residues), 90.9 bits, see alignment E=1.9e-29 amino acids 420 to 575 (156 residues), 31.5 bits, see alignment E=2.1e-11 amino acids 622 to 662 (41 residues), 26.4 bits, see alignment 7.3e-10 PF09334: tRNA-synt_1g" amino acids 38 to 171 (134 residues), 69.3 bits, see alignment E=7.1e-23 PF13603: tRNA-synt_1_2" amino acids 220 to 406 (187 residues), 231.1 bits, see alignment E=1.8e-72 PF08264: Anticodon_1" amino acids 704 to 826 (123 residues), 67.5 bits, see alignment E=3.2e-22

Best Hits

Swiss-Prot: 86% identical to SYL_BURM1: Leucine--tRNA ligase (leuS) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 86% identity to bmj:BMULJ_00505)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYN6 at UniProt or InterPro

Protein Sequence (863 amino acids)

>BPHYT_RS16785 leucyl-tRNA synthetase (Burkholderia phytofirmans PsJN)
MHEKYVPSDVESAAQGQWRAIDAYKSTETTDKPKFYCVSMLPYPSGKLHMGHVRNYTIND
VMYRYLRMNGYNVLMPMGWDAFGMPAENAAMANNVPPAKWTYDNIAYMKKQMQSMGLAID
WSREVATCSPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
ALVEKREIPMYYMRITQYADELLNDLEGLGWPERVKIMQQNWIGKSFGVNFGFPYEIDGE
QKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAKDKPELQAFIEECKRGGVAEADVATME
KKGMATGFTVTHPLTQEQVEVWIGNYVLMSYGEGAVMGVPAHDERDFAFVKKYGLPIKQV
VAVEGKEYSTDAWQEWYADKTGTLINSGKYDGLGYEAAVDQIAADLKELGLGDKQITWRL
RDWGVSRQRYWGTPIPIIHCPTCGDVPVPEKDLPVVLPEDLVPDGTGNPLARSEAFVNCT
CPTCGGAAKRETDTMDTFVDSSWYFYRYASPDAKTMVDERTDYWAPMDQYIGGIEHAILH
LLYSRFWAKVMRDMGLVNFGEPAKNLLTQGMVLNETYYRENEAGKKTWYNPADVTVSFDD
KGRPVGAVLNEDGQPVVLGGVEKMSKSKNNGVDPQLLIDQHGADTARLFVMFAAPPEQSL
EWSGSGVEGASRFLRRVWSFSQANEAALRQGGTFDAAQLSDVEKTLRREIYSVLKQADFD
YQRLQYNTVVSAAMKMLNALDSAKGARPAVLRETHSVLLRVLYPVVPHLTFQLWQELGYA
DELGSLLDAPWPKVDEKALEQSEIELVLQVNGKVRGAITVAKEASREAIEQLAAAHEMVA
KFSEGKAPKKIVVVPGRLVNVVV