Protein Info for BPHYT_RS16050 in Burkholderia phytofirmans PsJN

Updated annotation (from data): xylitol 2-dehydrogenase (EC 1.1.1.9)
Rationale: Specifically important for: Xylitol. (SEED_correct)
Original annotation: iditol 2-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 PF08240: ADH_N" amino acids 35 to 159 (125 residues), 91.7 bits, see alignment E=3.3e-30 PF00107: ADH_zinc_N" amino acids 197 to 325 (129 residues), 82.2 bits, see alignment E=3.2e-27

Best Hits

Swiss-Prot: 49% identical to ELTD_MYCS2: Erythritol/L-threitol dehydrogenase (eltD) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: K00008, L-iditol 2-dehydrogenase [EC: 1.1.1.14] (inferred from 100% identity to bpy:Bphyt_3233)

MetaCyc: 49% identical to erythritol/L-threitol dehydrogenase (Mycolicibacterium smegmatis)
1.1.1.M29 [EC: 1.1.1.M29]; 1.1.1.M28 [EC: 1.1.1.M29, 1.1.1.M28]; D-xylulose reductase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9]; Ribitol 2-dehydrogenase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9, 1.1.1.56]; L-arabinitol 4-dehydrogenase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9, 1.1.1.56, 1.1.1.12]

Predicted SEED Role

"Xylitol dehydrogenase (EC 1.1.1.9)" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 1.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.14

Use Curated BLAST to search for 1.1.1.12 or 1.1.1.14 or 1.1.1.56 or 1.1.1.9 or 1.1.1.M28 or 1.1.1.M29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SY89 at UniProt or InterPro

Protein Sequence (365 amino acids)

>BPHYT_RS16050 xylitol 2-dehydrogenase (EC 1.1.1.9) (Burkholderia phytofirmans PsJN)
MTTQSTDHDQQNMTAIVCHAPKDYRVEQVSKPRAGAHELVIRIAACGICASDCKCHSGAK
MFWGGPSPWVKAPVIPGHEFFGFVEEIGEGAADHFGVKMGDRVIAEQIVPCGKCRYCKSG
QYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISLEDAAIIEPLACAIHTVN
RGEVQLDDVVVIAGAGPLGLMMTQIAHLKTPKKLVVIDLVEERLALAREYGADVTINPKQ
DDALAIIHSLTDGYGCDVYIETTGAPIGVNQGMDLIRKLGRFVEFSVFGADTTLDWSVIG
DRKELDVRGAHLGPYCYPIAIDLLARGLVTSKGIVTHGFSLEEWDEAIKIANSLDSIKVL
LKPRA