Protein Info for BPHYT_RS15265 in Burkholderia phytofirmans PsJN

Annotation: DNA polymerase III subunit epsilon

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 PF00929: RNase_T" amino acids 16 to 163 (148 residues), 106.8 bits, see alignment E=1.7e-34 TIGR00573: exonuclease, DNA polymerase III, epsilon subunit family" amino acids 18 to 170 (153 residues), 77.6 bits, see alignment E=4.7e-26 PF01541: GIY-YIG" amino acids 210 to 282 (73 residues), 25.4 bits, see alignment E=1.4e-09

Best Hits

KEGG orthology group: K02342, DNA polymerase III subunit epsilon [EC: 2.7.7.7] (inferred from 100% identity to bpy:Bphyt_3077)

Predicted SEED Role

"DNA polymerase III epsilon subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T6A5 at UniProt or InterPro

Protein Sequence (383 amino acids)

>BPHYT_RS15265 DNA polymerase III subunit epsilon (Burkholderia phytofirmans PsJN)
MSEQFLPEPALDGPIVFVDLETTGGSTAEHRITEVGVVEIGPAGVSRWSSLVDPQQPIPS
FIQQLTGITNAMVRGAPTFDVIAPELFKRLNGKLFVAHNASFDRGFLRGEFRRVGLAFDP
DVLCTVRLSRALFPAEKRHGLDALVERHALVPSDRHRALADADLIWQFWQRLHGLVPLDV
LRTQIERTTRRYRLAGDITEDLLDTAPAGCGVYAFYGEEDLPLYVGRSVRVRQRLRSHLT
GERRSSKDIRLAQQVRRVEWRATGGELGAMLAEAQWIATLRPGHNRVPRVAKSDPADAPW
PFDGPIVFEEREEASQARAFHVVDRWCYVGHAASLALAAELHASSAAGPFELSTYRILQS
HLARGLRVMPLSVPIGAAVASLA